Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Thanyaburi, Pathum Thani, 12110, Thailand.
Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Thanyaburi, Pathum Thani, 12110, Thailand.
Anal Chim Acta. 2023 Aug 1;1267:341377. doi: 10.1016/j.aca.2023.341377. Epub 2023 May 15.
In this work, copper oxide nanoparticles (CuONPs) nanozymes paper-based analytical device was designed for the rapid detection of organophosphate pesticides in fruits and vegetables. The paper-based analytical device was modified with silica oxide nanoparticles to enhance the assay sensitivity. CuO nanozymes displayed peroxidase-like activity and catalyzed the oxidation of o-dianisidine in the presence of HO from the hydrolysis of acetylthiocholine. This results in the formation of a brown-colored product. In the presence of organophosphate pesticides such as malathion, acetylcholinesterase activity was inhibited, resulting in reduced color intensity production, and which was measured with a smartphone. The proposed nanozymes paper-based analytical device exhibited a good linear detection range (0.1-5 mg L), a low detection limit of 0.08 mg L, and the analysis time was only about 10 min for malathion detection under optimal conditions. Moreover, the CuONPs had excellent catalytic activity and higher stability than peroxidase. Finally, this device can be applied to detect organophosphate pesticides in fruits and vegetables with rapidity, accuracy, portability, and ease of handling in the field.
在这项工作中，设计了一种氧化铜纳米粒子（CuONPs）纳米酶纸基分析装置，用于快速检测水果和蔬菜中的有机磷农药。通过用氧化硅纳米粒子对纸基分析装置进行修饰，提高了分析灵敏度。CuO 纳米酶具有过氧化物酶样活性，并在 HO 的存在下催化邻二茴香胺的氧化，HO 来自乙酰硫代胆碱的水解。这导致形成棕色产物。在存在马拉硫磷等有机磷农药的情况下，乙酰胆碱酯酶的活性被抑制，导致颜色强度产物减少，并且可以用智能手机进行测量。在最佳条件下，该纳米酶纸基分析装置对马拉硫磷的检测表现出良好的线性检测范围（0.1-5 mg L）、低检测限为 0.08 mg L，分析时间仅约 10 分钟。此外，CuONPs 具有比过氧化物酶更高的催化活性和更高的稳定性。最后，该装置可用于快速、准确、便携和易于现场操作地检测水果和蔬菜中的有机磷农药。
