Takahashi M, Fujiwara M, Kishi K, Sakai C, Sanada M, Moriyama Y, Shibata A
Int J Cell Cloning. 1985 Sep;3(5):294-303. doi: 10.1002/stem.5530030502.
Due to an increase in mature neutrophils, a 72-year-old female with gall bladder cancer showed leukocytosis of up to 1.32 X 10(11)/l; hypercalcemia was up to 7.7 mEq/l in her terminal stage. Leukocyte counts and calcium values increased as the tumor progressed. There was no sign of infection or bone marrow metastasis. Cultured cells from the tumor tissue produced high colony-stimulating factor (CSF) activity into the supernatant. The tumor cell-conditioned medium stimulated exclusively granulocytic colonies. The study of this patient shows that leukocytosis was caused by the CSF produced by tumor cells. Approximately 90% of CSF activity was lost by heat treatment at 60 degrees C for 30 min. The CSF was stable over a pH range of 3-11 and was inactivated by treatment with proteolytic enzymes, but was not affected by treatment with DNase or neuraminidase. Molecular weight of the CSF, demonstrated by fractionation using Sephacryl S-200, was approximately 27,000 to 30,000.
一名72岁的胆囊癌女性因成熟中性粒细胞增多，白细胞计数高达1.32×10¹¹/l；终末期血钙高达7.7 mEq/l。随着肿瘤进展，白细胞计数和血钙值升高。无感染或骨髓转移迹象。肿瘤组织培养细胞向上清液中分泌高集落刺激因子（CSF）活性。肿瘤细胞条件培养基仅刺激粒细胞集落。对该患者的研究表明，白细胞增多是由肿瘤细胞产生的CSF引起的。60℃热处理30分钟使约90%的CSF活性丧失。CSF在pH 3-11范围内稳定，经蛋白水解酶处理失活，但不受DNA酶或神经氨酸酶处理的影响。使用Sephacryl S-200分级分离显示，CSF的分子量约为27,000至30,000。
