Department of Obstetrics and Gynaecology, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Guangdong Province, China.
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
J Reprod Immunol. 2024 Sep;165:104298. doi: 10.1016/j.jri.2024.104298. Epub 2024 Jul 7.
This study aims to conduct a preliminary exploration of the correlation between the oral microbiota of full-term pregnant women and both local placental immunity and the systemic immune system of the mother.
A total of 26 pregnant women participated in this study, with samples collected from oral swabs, placental tissue, and peripheral venous blood. High-throughput sequencing was used to examine the oral microbial community. Flow cytometry was employed to assess immune cells in placental tissue and peripheral venous blood. ELISA and Luminex liquid bead chip technology were utilized to detect cytokines in both placental tissue and peripheral venous blood.
In placental tissue, The oral microbial community is primarily negatively correlated with placental CD3+CD4+CD8+T cells and positively correlated with placental IL-5. In the peripheral blood, The oral microbial community is primarily positively correlated with maternal systemic immune parameters, including CD3+CD4+ T cells and the CD4+/CD8+ ratio, as well as positively correlated with peripheral IL-18.
The oral microbiota of full-term pregnant women participates in the regulatory function of the maternal immune system. Meanwhile, the oral microbial community may also be an important factor mediating local immune regulation in the placenta.
本研究旨在初步探讨足月孕妇口腔微生物群与局部胎盘免疫和母体全身免疫系统之间的相关性。
共纳入 26 名孕妇，采集口腔拭子、胎盘组织和外周静脉血样本。采用高通量测序技术检测口腔微生物群落，采用流式细胞术检测胎盘组织和外周静脉血中的免疫细胞，采用 ELISA 和 Luminex 液相芯片技术检测胎盘组织和外周静脉血中的细胞因子。
在胎盘组织中，口腔微生物群落主要与胎盘 CD3+CD4+CD8+T 细胞呈负相关，与胎盘 IL-5 呈正相关。在外周血中，口腔微生物群落主要与母体全身免疫参数呈正相关，包括 CD3+CD4+T 细胞和 CD4+/CD8+比值，与外周血 IL-18 呈正相关。
足月孕妇的口腔微生物群参与了母体免疫系统的调节功能。同时，口腔微生物群落也可能是调节胎盘局部免疫的重要因素。
