Bassols Judit, Serino Matteo, Carreras-Badosa Gemma, Burcelin Rémy, Blasco-Baque Vincent, Lopez-Bermejo Abel, Fernandez-Real José-Manuel
Department of Pediatrics, Dr. Josep Trueta Hospital and Girona Institute for Biomedical Research, Girona, Spain.
Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse, France.
Pediatr Res. 2016 Dec;80(6):777-784. doi: 10.1038/pr.2016.155. Epub 2016 Aug 4.
The human microbiota is a modulator of the immune system. Variations in the placental microbiota could be related with pregnancy disorders. We profiled the placental microbiota and microbiome in women with gestational diabetes (GDM) and studied its relation to maternal metabolism and placental expression of anti-inflammatory cytokines.
Placental microbiota and microbiome and expression of anti-inflammatory cytokines (IL10, TIMP3, ITGAX, and MRC1MR) were analyzed in placentas from women with GDM and from control women. Fasting insulin, glucose, O'Sullivan glucose, lipids, and blood cell counts were assessed at second and third trimester of pregnancy.
Bacteria belonging to the Pseudomonadales order and Acinetobacter genus showed lower relative abundance in women with GDM compared to control (P < 0.05). In GDM, lower abundance of placental Acinetobacter associated with a more adverse metabolic (higher O'Sullivan glucose) and inflammatory phenotype (lower blood eosinophil count and lower placental expression of IL10 and TIMP3) (P < 0.05 to P = 0.001). Calcium signaling pathway was increased in GDM placental microbiome.
A distinct microbiota profile and microbiome is present in GDM. Acinetobacter has been recently shown to induce IL-10 in mice. GDM could constitute a state of placental microbiota-driven altered immunologic tolerance, making placental microbiota a new target for therapy in GDM.
人类微生物群是免疫系统的调节因子。胎盘微生物群的变化可能与妊娠疾病有关。我们分析了妊娠期糖尿病（GDM）女性的胎盘微生物群和微生物组，并研究了其与母体代谢及胎盘抗炎细胞因子表达的关系。
分析了GDM女性和对照女性胎盘的微生物群、微生物组以及抗炎细胞因子（IL10、TIMP3、ITGAX和MRC1MR）的表达。在妊娠中期和晚期评估空腹胰岛素、血糖、奥沙利文葡萄糖、血脂和血细胞计数。
与对照组相比，GDM女性中属于假单胞菌目和不动杆菌属的细菌相对丰度较低（P < 0.05）。在GDM中，胎盘不动杆菌丰度较低与更不利的代谢（更高的奥沙利文葡萄糖）和炎症表型（更低的血液嗜酸性粒细胞计数以及更低的胎盘IL10和TIMP3表达）相关（P < 0.05至P = 0.001）。GDM胎盘微生物组中钙信号通路增强。
GDM中存在独特的微生物群谱和微生物组。最近研究表明，不动杆菌可在小鼠中诱导IL-10。GDM可能构成一种由胎盘微生物群驱动的免疫耐受改变状态，使胎盘微生物群成为GDM治疗新靶点。
