Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electrophotonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, PR China.
Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electrophotonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 5;322:124810. doi: 10.1016/j.saa.2024.124810. Epub 2024 Jul 10.
Programmed cell death (PCD) is a controlled form of cell death and it plays an essential role in maintaining homeostasis. Golgi apparatus works as the hotspot during the early event of PCD and Golgi polarity, a vital microenvironment factor, can be regarded as an indicator of physiological status. Combined Golgi-targeted group phenylsulfonamide as electron acceptor group and triphenylamine as electron donor group, a novel Golgi-targeted fluorescent probe GTO had been developed. GTO showed good sensitivity and selectivity to polarity and its remarkable photostability makes it potentially useful for long-term cellular monitoring. In practice, GTO demonstrated good cell permeability and Golgi targeting capabilities. According to our results, GTO was applied to reveal the polarity increase during the early event of PCD and the encouraging results illustrated that GTO was an imaging tool for monitoring polarity in Golgi apparatus and the exploration in early diagnosis and drug discovery.
程序性细胞死亡（PCD）是一种细胞的可控死亡形式，它在维持体内平衡方面起着至关重要的作用。高尔基体在 PCD 的早期事件中充当热点，而高尔基体极性作为一个重要的微环境因素，可以被视为生理状态的一个指标。将靶向高尔基体的苯基磺酰胺作为电子受体基团和三苯基胺作为电子给体基团相结合，开发了一种新型的靶向高尔基体的荧光探针 GTO。GTO 对极性具有良好的灵敏度和选择性，其显著的光稳定性使其在长期细胞监测中具有潜在的应用价值。在实际应用中，GTO 表现出良好的细胞通透性和靶向高尔基体的能力。根据我们的结果，GTO 被应用于揭示 PCD 早期事件中极性的增加，令人鼓舞的结果表明，GTO 是一种用于监测高尔基体中极性的成像工具，可用于早期诊断和药物发现的探索。
