College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education , Shandong Normal University , Jinan 250014 , People's Republic of China.
Anal Chem. 2019 Mar 5;91(5):3382-3388. doi: 10.1021/acs.analchem.8b04703. Epub 2019 Feb 21.
Depression is associated with decreased expression of brain-derived neurotrophic factor (BDNF) which assembled in Golgi apparatus. The changes might be closely related to variation in Golgi apparatus polarity. Thus, developing a nondestructive method to detect polarity in living cells and in vivo can facilitate accurate diagnosis and prognosis of depression. Herein, we created a new near-infrared Golgi-targetable fluorescent probe (Golgi-P) in which the merocyanine and benzoyl difluoroboronate moieties sense polarity changes. Golgi-P exhibited a decrease in fluorescence intensity and red-shift of maximum emission wavelength as the increase in polarity. Using Golgi-P, we discovered distinctly higher polarity in brains of mice with depression phenotype for the first time. Furthermore, our results disclosed that the elevation of polarity could due to the reduced synthesis of BDNF. Altogether, this study offers a new strategy for the accurate diagnosis of depression.
抑郁症与脑源性神经营养因子（BDNF）的表达减少有关，BDNF 聚集在高尔基体内。这些变化可能与高尔基器极性的变化密切相关。因此，开发一种非破坏性的方法来检测活细胞和体内的极性，可以促进抑郁症的准确诊断和预后。在此，我们创建了一种新的近红外高尔基靶向荧光探针（Golgi-P），其中的甲川和苯甲酰二氟硼酸盐部分感知极性变化。随着极性的增加，Golgi-P 的荧光强度降低，最大发射波长红移。使用 Golgi-P，我们首次发现抑郁表型小鼠大脑中的极性明显升高。此外，我们的结果表明，极性的升高可能是由于 BDNF 的合成减少所致。总的来说，这项研究为抑郁症的准确诊断提供了一种新策略。
