School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
Bioorg Chem. 2022 May;122:105741. doi: 10.1016/j.bioorg.2022.105741. Epub 2022 Mar 14.
When the cell environment changes or is stimulated, the Golgi apparatus will respond to the corresponding stress, through the opening of related pathways, the expression of corresponding substances can be promoted or inhibited to achieve the purpose of controlling cell redox homeostasis and reducing cytotoxicity. Intuitive analysis of the changes in the content of various substances in the process of stress has important guiding value for the further study of stress response, drug evaluation and clinical diagnosis. Therefore, for the Cys overexpressed during the oxidative stress of the Golgi apparatus, we developed a specific and sensitive fluorescent probe (Gol-NCS) to visually monitor the biologically important Cys in real time. The probe has low cytotoxicity and shows great potential in cell and zebrafish imaging, it can detect the changes of endogenous and exogenous cysteine. It is important to explore the synthetic pathway of Cys during Golgi stress by using the Golgi targeting performance of the probe Gol-NCS. It is confirmed by fluorescence imaging for the first time that the activity of CSE enzyme plays a decisive role in the formation of Cys. Therefore, probe Gol-NCS with excellent photochemical properties is expected to provide help for the research on the involvement of Cys in Golgi stress.
当细胞环境发生变化或受到刺激时，高尔基体将对相应的应激作出反应，通过开放相关途径，可以促进或抑制相应物质的表达，从而达到控制细胞氧化还原稳态和降低细胞毒性的目的。直观分析应激过程中各种物质含量的变化，对进一步研究应激反应、药物评价和临床诊断具有重要的指导价值。因此，对于高尔基体氧化应激过程中过表达的 Cys，我们开发了一种特异性和灵敏性的荧光探针（Gol-NCS），以实时可视化监测重要的生物 Cys。该探针具有低细胞毒性，在细胞和斑马鱼成像中显示出巨大的潜力，可检测内源性和外源性半胱氨酸的变化。利用探针 Gol-NCS 的高尔基体靶向性能来探索 Cys 在高尔基体应激过程中的合成途径非常重要。荧光成像首次证实，CSE 酶的活性对半胱氨酸的形成起着决定性的作用。因此，具有优异光化学性质的探针 Gol-NCS 有望为研究 Cys 参与高尔基体应激提供帮助。
