Department of Analytical Chemistry, Faculty of Chemistry, Alzahra University, Vanak, Tehran, Iran.
Analytical and Bioanalytical Research Centre, Alzahra University, Vanak, Tehran, Iran.
Sci Rep. 2024 Jul 13;14(1):16192. doi: 10.1038/s41598-024-66355-9.
Quantifying small amounts of the 17-hydroxyprogesterone in various matrix is crucial for different purposes. In this study, a commercial polydimethylsiloxane stir bar was used to extract hormone from water and urine samples. Analysis was performed by high-performance liquid chromatography using a UV detector. The response surface methodology was used to optimize the desorption and extraction steps, with predicted optimal point relative errors of 1.25% and 6.40%, respectively. The optimized method was validated with a linear range of 1.21-1000.00 for aqueous and 2.43-2000.00 ng mL for urine samples. The coefficient of determination was 0.9998 and 0.9967, and the detection limit of the proposed method was obtained to be 0.40 and 0.80 ng mL for aqueous and urine samples, respectively. The recovery percentage and relative standard deviation within a day and between three days after the addition of three different concentration levels of the standard to the control sample were 87-103% and 0.4-3.6% for aqueous and 87.5-101% and 0.1-5.2% for urine samples, respectively. The results show that the proposed method can be appropriate and cost-effective for extracting and analyzing this hormone. In addition, using three different tools, the greenness of the proposed method was proven.
定量分析各种基质中少量的 17-羟孕酮对于不同的目的至关重要。在这项研究中，使用商业聚二甲基硅氧烷搅拌棒从水和尿液样品中提取激素。采用高效液相色谱法，用紫外检测器进行分析。响应面法用于优化解吸和提取步骤，预测最优点相对误差分别为 1.25%和 6.40%。优化后的方法在水相的线性范围为 1.21-1000.00ngmL 和尿样的线性范围为 2.43-2000.00ngmL 时进行了验证。决定系数分别为 0.9998 和 0.9967，建议方法的检测限分别为 0.40 和 0.80ngmL 水相和尿样。在向对照样品中加入三个不同浓度水平的标准品后，一天内和三天内的回收率和相对标准偏差分别为 87-103%和 0.4-3.6%水相和 87.5-101%和 0.1-5.2%尿样。结果表明，该方法适用于提取和分析这种激素，且经济有效。此外，使用三种不同的工具证明了所提出方法的绿色性。
