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长链非编码 RNA PITPNA-AS1 介导 miR-129-5p 在前列腺癌中的诊断潜力。

LncRNA PITPNA-AS1 mediates the diagnostic potential of miR-129-5p in prostate cancer.

机构信息

Department of Urology Surgery, Jiaozhou Central Hospital of Qingdao, Shandong, 266300, China.

Medical School of University of Electronic Science and Technology of China, Chengdu, 610051, China.

出版信息

BMC Urol. 2024 Jul 13;24(1):146. doi: 10.1186/s12894-024-01528-2.

DOI:10.1186/s12894-024-01528-2
PMID:39003446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11245843/
Abstract

BACKGROUND

LncRNA has an effective value in many diseases, which has long been applied in the diagnosis, treatment and prognosis of prostate cancer. This study focused on lncRNA PITPNA-AS1, and its diagnostic potential in prostate cancer has been explored.

METHODS

The expression of PITPNA-AS1 and miR-129-5p in prostate cancer serum and sample cells was determined by real-time quantitative polymerase chain reaction (RT-qPCR). The relationship between the expression of PITPNA-AS1 and clinicopathological parameters was considered. ROC curve prompted the diagnostic value of PITPNA-AS1. The effect of PITPNA-AS1 on prostate cancer cells was verified using vitro cells assay. Luciferase activity assay and RIP assay demonstrated the sponge relationship of PITPNA-AS1 to miR-129-5p.

RESULTS

PITPNA-AS1 level was increased, while miR-129-5p was obviously decreased in prostate cancer. PITPNA-AS1 expression was associated with Gleason grade, lymph node metastasis and TNM stage in patients. The area under the curve (AUC) was 0.910, with high sensitivity and specificity. PITPNA-AS1 was elucidated to directly target miR-129-5p, whereas silencing PITPNA-AS1 negatively affected prostate cancer cell proliferation, migration and invasion. Intervention of miR-129-5p inhibitor reversed the effect of silencing PITPNA-AS1 on cells.

CONCLUSIONS

PITPNA-AS1 was relatively highly expressed in prostate cancer and mediated the pathophysiological process of patients, which may serve as a diagnostic indicator. Silencing of the PITPNA-AS1 sponge miR-129-5p inhibited the biological function of the cells, indicating that PITPNA-AS1 may represent a novel therapeutic target for prostate cancer.

摘要

背景

LncRNA 在许多疾病中具有重要价值，长期以来一直应用于前列腺癌的诊断、治疗和预后。本研究聚焦于 lncRNA PITPNA-AS1，并探讨其在前列腺癌中的诊断潜力。

方法

采用实时定量聚合酶链反应（RT-qPCR）检测前列腺癌血清和样本细胞中 PITPNA-AS1 和 miR-129-5p 的表达。考虑 PITPNA-AS1 表达与临床病理参数的关系。ROC 曲线提示 PITPNA-AS1 的诊断价值。通过体外细胞实验验证 PITPNA-AS1 对前列腺癌细胞的影响。荧光素酶活性测定和 RIP 测定证实了 PITPNA-AS1 对 miR-129-5p 的海绵关系。

结果

前列腺癌中 PITPNA-AS1 水平升高，miR-129-5p 明显降低。PITPNA-AS1 表达与患者的 Gleason 分级、淋巴结转移和 TNM 分期有关。曲线下面积（AUC）为 0.910，具有较高的敏感性和特异性。PITPNA-AS1 被证实可直接靶向 miR-129-5p，而沉默 PITPNA-AS1 可负向影响前列腺癌细胞的增殖、迁移和侵袭。miR-129-5p 抑制剂的干预逆转了沉默 PITPNA-AS1 对细胞的影响。

结论

PITPNA-AS1 在前列腺癌中相对高表达，并介导患者的病理生理过程，可能作为诊断指标。沉默 PITPNA-AS1 海绵 miR-129-5p 抑制了细胞的生物学功能，表明 PITPNA-AS1 可能成为前列腺癌的新治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/d284d7cb1f27/12894_2024_1528_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/5143f38eb638/12894_2024_1528_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/dd145d897703/12894_2024_1528_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/c818bcdac035/12894_2024_1528_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/d284d7cb1f27/12894_2024_1528_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/5143f38eb638/12894_2024_1528_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/dd145d897703/12894_2024_1528_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/c818bcdac035/12894_2024_1528_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd94/11245843/d284d7cb1f27/12894_2024_1528_Fig4_HTML.jpg

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