Departamento de Producción Animal, Facultad de Veterinaria, Universidad de León, León, Spain.
Anim Biotechnol. 2024 Jun 28;35(1):2374328. doi: 10.1080/10495398.2024.2374328. Epub 2024 Jul 14.
Long non-coding RNAs (lncRNAs) are being studied in farm animals due to their association with traits of economic interest, such as fat deposition. Based on the analysis of perirenal fat transcriptomes, this research explored the relevance of these regulatory elements to fat deposition in suckling lambs. To that end, meta-analysis techniques have been implemented to efficiently characterize and detect differentially expressed transcripts from two different RNA-seq datasets, one including samples of two sheep breeds that differ in fat deposition features, Churra and Assaf ( = 14), and one generated from Assaf suckling lambs with different fat deposition levels ( = 8). The joint analysis of the 22 perirenal fat RNA-seq samples with the software allowed the detection of 3953 novel lncRNAs. After the meta-analysis, 251 differentially expressed genes were identified, 21 of which were novel lncRNAs. Additionally, a co-expression analysis revealed that, in suckling lambs, lncRNAs may play a role in controlling angiogenesis and thermogenesis, processes highlighted in relation to high and low fat deposition levels, respectively. Overall, while providing information that could be applied for the improvement of suckling lamb carcass traits, this study offers insights into the biology of perirenal fat deposition regulation in mammals.
长链非编码 RNA（lncRNA）因其与经济性状（如脂肪沉积）相关而在农场动物中受到研究。本研究基于对肾周脂肪转录组的分析，探讨了这些调节元件与哺乳期羔羊脂肪沉积的相关性。为此，实施了荟萃分析技术，以有效地对两个不同 RNA-seq 数据集的差异表达转录本进行特征分析和检测，一个数据集包括脂肪沉积特征不同的两个绵羊品种（Churra 和 Assaf）的样本（n=14），另一个数据集则来源于脂肪沉积水平不同的 Assaf 哺乳期羔羊（n=8）。通过对 22 个肾周脂肪 RNA-seq 样本与 软件进行联合分析，共检测到 3953 个新的 lncRNA。荟萃分析后，共鉴定出 251 个差异表达基因，其中 21 个是新的 lncRNA。此外，共表达分析表明，在哺乳期羔羊中，lncRNA 可能在控制血管生成和生热过程中发挥作用，这两个过程分别与高和低脂肪沉积水平有关。总的来说，本研究为改善哺乳羔羊胴体性状提供了信息，同时也深入了解了哺乳动物肾周脂肪沉积调节的生物学。
