Department of Laboratory Medicine, Jeonbuk National University Medical School and Hospital, Jeonju, Republic of Korea.
Research Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Republic of Korea.
Curr Microbiol. 2024 Jul 14;81(9):269. doi: 10.1007/s00284-024-03798-3.
The escalation of antimicrobial resistance (AMR) due to the excessive and inappropriate use of antimicrobials has prompted the urgent need for more rapid and effective antimicrobial susceptibility testing (AST) methods. Conventional AST techniques often take 16-24 h, leading to empirical prescription practices and the potential emergence of AMR. The study aimed to develop a rapid disk diffusion (RDD) method utilizing laser speckle formation (LSF) technology to expedite AST results. The study aimed to evaluate the performance of LSF technology in determining antimicrobial susceptibility. In this study, preclinical and clinical settings were established to compare the LSF technology with conventional disk diffusion (DD) methods to measure the inhibition zones. Preclinical experiments with different bacterial strains demonstrated more than 70% categorical agreement (CA) against most antimicrobials. Further, clinical experiments with multiple strains and antibiotics revealed CA ranging from 40 to 79%, while major and minor discrepancies were observed around 30% and 11%, respectively. These observations revealed high concordance between RDD and DD for multiple antimicrobials in multiple species. The results underscore the potential of RDD-based LSF technology for hastening AST procedures. The current study is marked by a unique equipment setup and analysis approach. Collectively, the suggested laser-based RDD showed greater potential than previously developed comparable methods. The proposed method and design have a higher application potential than formerly developed similar technologies. Together, the study contributes to the ongoing development of rapid AST methods.
由于抗菌药物的过度和不适当使用导致抗菌药物耐药性（AMR）的升级，迫切需要更快速和有效的抗菌药物敏感性测试（AST）方法。传统的 AST 技术通常需要 16-24 小时，导致经验性处方实践和 AMR 的出现。本研究旨在开发一种利用激光散斑形成（LSF）技术的快速圆盘扩散（RDD）方法，以加快 AST 结果。本研究旨在评估 LSF 技术在确定抗菌药物敏感性方面的性能。在本研究中，建立了临床前和临床环境，以比较 LSF 技术与传统的圆盘扩散（DD）方法来测量抑制区。针对不同细菌菌株的临床前实验表明，超过 70%的分类一致性（CA）对大多数抗菌药物。此外，对多种菌株和抗生素进行的临床实验显示 CA 范围在 40%至 79%之间，而主要和次要差异分别约为 30%和 11%。这些观察结果表明，RDD 和 DD 对多种抗菌药物在多个物种中的一致性很高。基于 RDD 的 LSF 技术具有加速 AST 程序的潜力。本研究的特点是独特的设备设置和分析方法。总的来说，基于激光的 RDD 比以前开发的类似方法显示出更大的潜力。与以前开发的类似技术相比，所提出的方法和设计具有更高的应用潜力。该研究为快速 AST 方法的不断发展做出了贡献。
