Department of Biotechnology and Food Engineering, MCI - The Entrepreneurial School, Maximilianstrasse 2, 6020, Innsbruck, Austria.
Department of Environmental, Process and Energy Engineering, MCI - The Entrepreneurial School, Maximilianstrasse 2, 6020, Innsbruck, Austria.
Bioprocess Biosyst Eng. 2024 Oct;47(10):1669-1682. doi: 10.1007/s00449-024-03060-x. Epub 2024 Jul 14.
Bio-based production of silver nanoparticles represents a sustainable alternative to commercially applied physicochemical manufacturing approaches and provides qualitatively highly valuable nanomaterials due to their narrow size dispersity, high stability and biocompatibility with broad application potentials. The intrinsic features of nanoparticles depend on size and shape, whereby the controlled synthesis is a challenging necessity. In the present study, the biosynthesis of size-tuned silver nanoparticles based on cell-free extracts of Saccharomyces cerevisiae DSM 1333 was investigated. Single parameter optimization strategies in phases of cultivation, extraction, and synthesis were performed to modify the nanoparticle scale and yield. Visible light was exploited as a tool in nanoparticle production. The influence of white light on the biosynthesis of silver nanoparticles was determined by using novel LED systems with the exposition of varying irradiation intensities and simultaneous performance of control experiments in the dark. Characterization of the resulting nanomaterials by spectrophotometric analysis, dynamic light scattering, scanning electron microscopy, and energy dispersive X-ray spectroscopy, revealed spherical silver nanoparticles with controlled, light-mediated size shifts in markedly increased quantities. Matching of irradiated and non-irradiated reaction mixtures mirrored the enormous functionality of photon input and the high sensitivity of the biosynthesis process. The silver nanoparticle yields increased by more than 90% with irradiation at and the reduction of particle dimensions was achieved with significant shifts of size-specific absorption maxima from 440 to 410 nm, corresponding to particle sizes of 130 nm and 100 nm, respectively. White light emerged as an excellent tool for nano-manufacturing with advantageous effects for modulating unique particle properties.
基于生物的银纳米颗粒生产代表了对商业应用物理化学制造方法的可持续替代，并且由于其窄的尺寸分散性、高稳定性和与广泛应用潜力的生物相容性，提供了具有高质量价值的纳米材料。纳米颗粒的固有特性取决于尺寸和形状，因此可控合成是一项具有挑战性的必要性。在本研究中，研究了基于酿酒酵母 DSM 1333 的无细胞提取物的大小可调的银纳米颗粒的生物合成。在培养、提取和合成阶段进行了单参数优化策略，以改变纳米颗粒的规模和产率。可见光被用作纳米颗粒生产的工具。通过使用具有不同辐照度的新型 LED 系统并同时在黑暗中进行对照实验，确定了白光对银纳米颗粒生物合成的影响。通过分光光度分析、动态光散射、扫描电子显微镜和能量色散 X 射线光谱对所得纳米材料进行了表征，结果表明，银纳米颗粒呈球形，通过受控的、受光介导的尺寸变化，数量显著增加。辐照和非辐照反应混合物的匹配反映了光子输入的巨大功能和生物合成过程的高灵敏度。在 下辐照时，银纳米颗粒的产率增加了 90%以上，并且通过显著的吸收最大值从 440nm 到 410nm 的尺寸特异性位移实现了颗粒尺寸的减小，分别对应于 130nm 和 100nm 的颗粒尺寸。白光作为纳米制造的一种极好工具，具有调节独特颗粒性质的有利效果。
