Lim Hyun-Ah, Mishra Amrita, Yun Soon-Il
Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 561-756, South Korea.
J Nanosci Nanotechnol. 2011 Jan;11(1):518-22. doi: 10.1166/jnn.2011.3266.
In the present study, the synthesis of gold and silver nanoparticles was investigated using the culture supernatant broth of the yeast Saccharomyces cerevisae. Gold nanoparticles were formed within 24 hours of gold ion coming in contact with the culture supernatant broth. In case of silver the reduction process took 48 hours. The synthesized nanoparticles were investigated by UV-Visible spectroscopy. Distinct surface plasmon peaks were observed at 540 nm and 415 nm for gold and silver nanoparticles respectively. Bio-TEM micrographs of the synthesized nanoparticles indicated that the particles were well dispersed and near spherical in shape. The size range of the gold and silver nanoparticles was around 20-100 nm and 5-20 nm respectively. XRD patterns showed the presence of three distinct peaks corresponding to gold and silver nanoparticles respectively. A pH range of 4 to 6 and 8 to 10 favored optimum synthesis of gold and silver nanoparticles respectively. The process of reduction being extra cellular could be used in future for downstream processing in an eco friendly manner.
在本研究中，利用酿酒酵母的培养上清液对金和银纳米颗粒的合成进行了研究。金离子与培养上清液接触后24小时内形成了金纳米颗粒。对于银来说，还原过程需要48小时。通过紫外可见光谱对合成的纳米颗粒进行了研究。分别在540 nm和415 nm处观察到金和银纳米颗粒明显的表面等离子体峰。合成纳米颗粒的生物透射电子显微镜图像表明，颗粒分散良好且形状接近球形。金和银纳米颗粒的尺寸范围分别约为20 - 100 nm和5 - 20 nm。X射线衍射图谱分别显示了对应于金和银纳米颗粒的三个不同峰。pH值范围为4至6和8至10分别有利于金和银纳米颗粒的最佳合成。还原过程在细胞外进行，未来可用于以环保方式进行下游加工。
