Arévalo J, Panebra A, Santa Cruz C
J Protozool. 1985 Aug;32(3):553-5. doi: 10.1111/j.1550-7408.1985.tb04061.x.
Simple culture conditions that allow good growth and high yields of trypomastigotes are described. The proportion of metacyclic trypomastigotes increases with the concentration of hemin in the culture medium, reaching a peak of 80% after 10 days with 20 mg hemin/liter.