克氏锥虫体外循环后期发育：一种简化程序

Metacyclogenesis of Trypanosoma cruzi in vitro: a simplified procedure.

作者信息

Sullivan J J

出版信息

Trans R Soc Trop Med Hyg. 1982;76(3):300-3. doi: 10.1016/0035-9203(82)90173-0.

Abstract

A simplified procedure for obtaining high levels of infective, metacyclic trypomastigotes of a Brazilian strain of Trypanosoma cruzi in vitro is described. Epimastigotes cultured in Yaeger's Liver Infusion Tryptose Medium, when transferred to Grace's Insect Tissue Culture Medium (GMA) supplemented with 10% foetal bovine serum, yielded over 90% metacyclic forms. Decreasing or eliminating the serum from GMA adversely affected growth but did not decrease levels of transformation, thus providing a chemically defined culture system for analysing factors influencing metacyclogenesis of T. cruzi.

摘要

本文描述了一种在体外获得高感染性的巴西克氏锥虫循环后期锥鞭毛体的简化方法。在耶格尔肝浸液胰蛋白胨培养基中培养的前鞭毛体，转移至添加10%胎牛血清的格雷斯昆虫组织培养基（GMA）后，产生了超过90%的循环后期形态。从GMA中减少或去除血清会对生长产生不利影响，但不会降低转化水平，从而为分析影响克氏锥虫循环后期发育的因素提供了一种化学成分明确的培养系统。