Aalseth E P, Saacke R G
J Reprod Fertil. 1985 Jul;74(2):473-8. doi: 10.1530/jrf.0.0740473.
Swelling of the apical ridge and anterior acrosome of motile bovine spermatozoa was observed during in-vitro storage using differential interference-contrast optics. This morphological alteration is different from that described as the false acrosome reaction on immotile spermatozoa, apparent in ageing semen samples and which has been associated with cell death. In this study, transmission electron microscopy revealed that the apical ridge acrosomal matrix was extended into complex folds and/or projections. Acrosomal and plasma membrane integrity was retained. Storing spermatozoa (1500 X 10(6)/ml) in seminal plasma at 4 degrees C for 1 day was most conducive to the swelling of the apical ridge. Replacing seminal plasma with egg yolk-citrate inhibited swelling. However, incubating semen at 37 degrees C in egg yolk-Tris-fructose extender (25 X 10(6) spermatozoa/ml) after storage in egg yolk-citrate at 4 degrees C for greater than or equal to 3 days restored the swelling characteristic.
使用微分干涉相差光学显微镜观察到，体外保存期间，活动的牛精子顶脊和顶体前部出现肿胀。这种形态学改变不同于在老化精液样本中不活动精子上所描述的假顶体反应，假顶体反应与细胞死亡有关。在本研究中，透射电子显微镜显示顶脊顶体基质延伸成复杂的褶皱和/或突起。顶体和质膜完整性得以保留。将精子（1500×10⁶/ml）于4℃保存在精浆中1天最有利于顶脊肿胀。用卵黄柠檬酸盐替代精浆可抑制肿胀。然而，在4℃于卵黄柠檬酸盐中保存≥3天后，将精液于37℃在卵黄-三羟甲基氨基甲烷-果糖稀释液（25×10⁶精子/ml）中孵育可恢复肿胀特征。
