Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen, 40002, Thailand; The Research and Development Network Center of Animal Breeding and Omics, Khon Kaen University, Khon Kaen, 40002, Thailand.
Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen, 40002, Thailand.
Theriogenology. 2024 Oct 1;227:31-40. doi: 10.1016/j.theriogenology.2024.07.008. Epub 2024 Jul 11.
The decrease in fertility in aging roosters is related to the reduced quality of ejaculated sperm. This study aimed to investigate the effect of dietary supplementation with ginseng extract at various concentrations (0-150 mg/kg) on testicular function, semen preservation, and fertility at different stages of sexual maturity (mature and aging roosters) in Thai native roosters. Pradu Hang Dum roosters at 32 (mature; n = 24) and 75 (aging; n = 24) weeks of age were fed diets with non-supplemented or supplemented ginseng extracts (50, 100, and 150 mg/kg) until the end of the experiment. In experiment 1, fresh semen samples were examined for the quality parameters of semen volume, sperm concentration, sperm motility, sperm viability, lipid peroxidation, and enzymatic activities. In experiment 2, semen was preserved at 5 °C for up to 48 h, and the semen quality and fertility potential were determined. In experiment 3, testicular function and testosterone concentrations were evaluated. The results showed that ginseng extract supplementation in the diets of both mature and aging roosters at 50 and 100 mg/kg improved fresh semen quality (P < 0.05). A decrease in malondialdehyde levels in fresh semen was observed with increasing enzyme activities. In mature roosters, the progressive motility of cold-stored semen and fertility rates were higher in the G50 and G100 groups compared to the control and G150 groups after 24 h of storage (P < 0.05). In aging roosters, the highest significant differences in progressive motility, viability, and fertility rates were observed in the G50 and G100 groups at all storage times (P < 0.01). These improvements might be attributed to good testicular function in spermatogenesis, as revealed by the results of histological examination and testosterone concentrations. However, higher doses of ginseng extract supplementation negatively affected sperm quality. In summary, the recommended dose of ginseng extract supplementation in diets is 50 mg/kg. Fertility results indicated that insemination with semen preserved for 24 h was satisfactory in both mature and aging roosters.
随着公鸡年龄的增长，其繁殖能力下降，与射出精液的质量下降有关。本研究旨在探讨不同浓度（0-150mg/kg）的人参提取物对泰国本地公鸡生殖功能、精液保存和不同性成熟阶段（成熟和衰老公鸡）的繁殖力的影响。32 周龄（成熟；n=24）和 75 周龄（衰老；n=24）的 Pradu Hang Dum 公鸡分别喂食添加或不添加人参提取物（50、100 和 150mg/kg）的饲料，直至实验结束。在实验 1 中，新鲜精液样本的精液体积、精子浓度、精子活力、精子存活率、脂质过氧化和酶活性等质量参数进行了检测。在实验 2 中，精液在 5°C 下保存长达 48 小时，然后检测精液质量和繁殖力。在实验 3 中，检测了睾丸功能和睾酮浓度。结果表明，成熟和衰老公鸡日粮中添加 50 和 100mg/kg 的人参提取物可提高新鲜精液质量（P<0.05）。随着酶活性的增加，新鲜精液中丙二醛水平降低。在成熟公鸡中，与对照组和 G150 组相比，G50 和 G100 组在 24 小时冷藏后，精子的前向运动能力和受精力更高（P<0.05）。在衰老公鸡中，在所有储存时间中，G50 和 G100 组的精子前向运动能力、活力和受精力均有显著提高（P<0.01）。这些改善可能归因于精子发生中良好的睾丸功能，这从组织学检查和睾酮浓度的结果中可以看出。然而，较高剂量的人参提取物补充对精子质量有负面影响。总之，日粮中人参提取物的推荐添加量为 50mg/kg。繁殖力结果表明，成熟和衰老公鸡的精液保存 24 小时后的授精效果令人满意。
