Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
J Glob Antimicrob Resist. 2024 Sep;38:227-230. doi: 10.1016/j.jgar.2024.07.001. Epub 2024 Jul 14.
To investigate the tet(X) gene, a determinant of tigecycline resistance, in the emerging pathogen Elizabethkingia meningoseptica and its association with an integrative and conjugative element (ICE).
All E. meningoseptica genomes from the National Center for Biotechnology Information (n = 87) were retrieved and annotated for resistome searches using the CARD database. A phylogenic analysis was performed based on the E. meningoseptica core genome. The ICE was identified through comparative genomics with other ICEs occurring in Elizabethkingia spp.
Phylogenetic analysis revealed E. meningoseptica genomes from six countries distributed across different lineages, some of which persisted for years. The common resistome of these genomes included bla, bla, bla, ranA/B, aadS, and catB (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (dfrA, ereD, bla, aadS, and tet(X)). Interestingly, tet(X) and aadS were located in an ICE of 49 769 bp (ICEEmSQ101), which was fully obtained from the E. meningoseptica SQ101 genome. We also showed evidence that the other 27 genomes harboured this ICE. The distribution of ICEEmSQ101, carrying tet(X), was restricted to a single Chinese lineage.
The tet(X) gene is not prevalent in the species E. meningoseptica, as previously stated for the genus Elizabethkingia, since it is present only in a single Chinese lineage. We identified that several E. meningoseptica genomes harboured an ICE that mobilized the Elizabethkingia tet(X) gene and exhibited characteristics similar to the ICEs of other Flavobacteria, which would favour their transmission in this bacterial family.
研究与替加环素耐药相关的 tet(X) 基因在新兴病原体脑膜败血伊丽莎白菌中的情况及其与整合子和转座子元件（ICE）的关系。
从国家生物技术信息中心（NCBI）中检索所有脑膜败血伊丽莎白菌基因组（n=87），并使用 CARD 数据库进行耐药组搜索注释。基于脑膜败血伊丽莎白菌核心基因组进行系统发育分析。通过与伊丽莎白菌属中其他 ICE 进行比较基因组学分析来鉴定 ICE。
系统发育分析显示来自六个国家的脑膜败血伊丽莎白菌基因组分布在不同的谱系中，其中一些谱系已经存在多年。这些基因组的常见耐药组包括 bla、bla、bla、ranA/B、aadS 和 catB（与β-内酰胺、氨基糖苷类和氯霉素耐药相关的基因）。一些基因组还存在其他耐药基因（dfrA、ereD、bla、aadS 和 tet(X)）。有趣的是，tet(X)和 aadS 位于一个 49769bp 的 ICE（ICEEmSQ101）中，该 ICE 完全从脑膜败血伊丽莎白菌 SQ101 基因组中获得。我们还证明了其他 27 个基因组也携带该 ICE。携带 tet(X)的 ICEEmSQ101 的分布仅限于中国的一个谱系。
与以前在伊丽莎白菌属中所述的情况一样，tet(X)基因在脑膜败血伊丽莎白菌属中并不普遍，因为它仅存在于一个中国谱系中。我们发现，一些脑膜败血伊丽莎白菌基因组携带一个可移动伊丽莎白菌 tet(X)基因的 ICE，并且具有类似于其他黄杆菌属 ICE 的特征，这将有利于它们在该细菌家族中的传播。
