Institute of Microbiology, University Hospital of Lausanne, Lausanne, Switzerland.
Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.
Int J Antimicrob Agents. 2017 Jan;49(1):93-97. doi: 10.1016/j.ijantimicag.2016.09.031. Epub 2016 Nov 15.
Elizabethkingia miricola is a Gram-negative non-fermenting rod emerging as a life-threatening human pathogen. The multidrug-resistant (MDR) carbapenemase-producing clinical isolate E. miricola EM_CHUV was recovered in the setting of severe nosocomial pneumonia. In this study, the genome of E. miricola EM_CHUV was sequenced and a functional analysis was performed, including a comparative genomic study with Elizabethkingia meningoseptica and Elizabethkingia anophelis. The resistome of EM_CHUV revealed the presence of a high number of resistance genes, including the presence of the bla and bla carbapenemase-encoding genes. Twelve mobility genes, with only two of them located in the proximity of resistance genes, and four potential genomic islands were identified in the genome of EM_CHUV, but no prophages or CRISPR sequences. Ten restriction-modification system (RMS) genes were also identified. In addition, we report the presence of a putative conjugative plasmid (pEM_CHUV) that does not encode any antibiotic resistance genes. Altogether, these findings point towards a limited number of DNA exchanges with other bacteria and suggest that multidrug resistance is an intrinsic trait of E. miricola owing to the presence of a high number of resistance genes within the bacterial core genome.
微杆菌属伊丽莎白菌是一种革兰氏阴性非发酵杆状菌，现已成为一种危及生命的人类病原体。多药耐药（MDR）碳青霉烯酶产生的临床分离株伊丽莎白菌 EM_CHUV 是在严重医院获得性肺炎的情况下回收的。在这项研究中，测序了 EM_CHUV 的基因组，并进行了功能分析，包括与脑膜败血伊丽莎白菌和嗜人按蚊伊丽莎白菌的比较基因组研究。EM_CHUV 的耐药组揭示了存在大量耐药基因，包括 bla 和 bla 碳青霉烯酶编码基因的存在。在 EM_CHUV 的基因组中鉴定了 12 个移动基因，其中只有两个位于耐药基因附近，并且还鉴定了四个潜在的基因组岛，但没有噬菌体或 CRISPR 序列。还鉴定了 10 个限制修饰系统（RMS）基因。此外，我们报告了存在一个推定的可接合质粒（pEM_CHUV），它不编码任何抗生素耐药基因。总之，这些发现表明与其他细菌的 DNA 交换数量有限，并表明多药耐药是 EM_CHUV 的固有特征，因为其细菌核心基因组中存在大量耐药基因。
