Department of Science and Technology, University of Sannio, via de Sanctis snc, 82100 Benevento, Italy.
Department of Science and Technology, University of Sannio, via de Sanctis snc, 82100 Benevento, Italy.
Sci Total Environ. 2024 Oct 20;948:174554. doi: 10.1016/j.scitotenv.2024.174554. Epub 2024 Jul 14.
This study investigates P. ostreatus and A. bisporus biodegradation capacity of low density polyethylene (LDPE) oxidised to simulate environmental weathering. Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) were used to analyse the degradation of LDPE treated with fungal cultures. Molecular implications of LDPE degradation by P. ostreatus and A. bisporus were evaluated by Reverse transcription followed by quantitative PCR (qRT-PCR) of lac, mnp and lip genes expression. After 90 days of incubation, FT-IR analysis showed, for both fungal treatments, an increasing in the intensity of peaks related to the asymmetric C-C-O stretching (1160 to 1000 cm) and the -OH stretching (3700 to 3200 cm) due to the formation of alcohols and carboxylic acid, indicating depolymerisation of LDPE. This was confirmed by the SEM analysis, where a widespread alteration of the surface morphology was observed for treated LDPE fragments. Results revealed that the exposure of P. ostreatus to oxidised LDPE treatment led to a significant increase in the expression of the lac6, lac7, lac9, lac10 and mnp2 genes, while A. bisporus showed an over-expression in lac2 and lac12 genes. The obtained results offer new perspectives for a biotechnological use of P. ostreatus and A. bisporus for plastic bioremediation.
本研究调查了糙皮侧耳（P. ostreatus）和双孢蘑菇（A. bisporus）对模拟环境风化的低密度聚乙烯（LDPE）的生物降解能力。傅里叶变换红外（FT-IR）光谱和扫描电子显微镜（SEM）用于分析真菌培养物处理后的 LDPE 降解情况。通过反转录定量 PCR（qRT-PCR）评估糙皮侧耳（P. ostreatus）和双孢蘑菇（A. bisporus）对 LDPE 的降解的分子影响，对 lac、mnp 和 lip 基因的表达进行了分析。在 90 天的孵育后，FT-IR 分析表明，对于两种真菌处理，与不对称 C-C-O 伸缩（1160 至 1000 cm）和 -OH 伸缩（3700 至 3200 cm）相关的峰强度增加，这是由于形成了醇和羧酸，表明 LDPE 的解聚。SEM 分析证实了这一点，其中处理后的 LDPE 片段的表面形貌广泛发生了改变。结果表明，糙皮侧耳（P. ostreatus）暴露于氧化 LDPE 处理会导致 lac6、lac7、lac9、lac10 和 mnp2 基因的表达显著增加，而双孢蘑菇（A. bisporus）则表现出 lac2 和 lac12 基因的过度表达。研究结果为糙皮侧耳（P. ostreatus）和双孢蘑菇（A. bisporus）在塑料生物修复中的生物技术应用提供了新的视角。
