Laboratory of Virology, Wageningen University & Research, Wageningen, The Netherlands; Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences, China.
Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Vaccine. 2024 Nov 14;42(25):126136. doi: 10.1016/j.vaccine.2024.07.037. Epub 2024 Jul 14.
Getah virus (GETV) is an emerging mosquito-borne virus with economic impact on the livestock industry in East Asia. In this study, we successfully produced GETV virus-like particles (VLPs) in insect cells using the baculovirus expression vector system. We show that the GETV envelope glycoproteins were successfully expressed at the surface of the insect cell and were glycosylated. VLPs were isolated from the culture fluid as enveloped particles of 60-80 nm in diameter. Two 1 µg vaccinations with this GETV VLP vaccine, without adjuvant, generated neutralizing antibody responses and protected wild-type C57/BL6 mice against GETV viremia and arthritic disease. The GETV VLP vaccine may find application as a horse and/or pig vaccine in the future.
克沙病毒（GETV）是一种新出现的虫媒病毒，对东亚的畜牧业有经济影响。在这项研究中，我们使用杆状病毒表达载体系统成功地在昆虫细胞中生产了 GETV 病毒样颗粒（VLPs）。我们表明，GETV 包膜糖蛋白成功地表达在昆虫细胞表面并发生了糖基化。VLPs 从培养液中分离出来，是直径为 60-80nm 的包膜颗粒。两次接种 1μg 这种 GETV VLP 疫苗，无需佐剂，即可产生中和抗体反应，并保护野生型 C57/BL6 小鼠免受 GETV 病毒血症和关节炎疾病的侵害。GETV VLP 疫苗未来可能会作为马和/或猪用疫苗得到应用。
