Department of Analytical Chemistry, Faculty of Chemistry, Alzahra University, Tehran, Iran.
Luminescence. 2024 Jul;39(7):e4828. doi: 10.1002/bio.4828.
The antibiotic tetracycline can be efficiently used as medicine for the deterrence of bacterial infections in humans, animals, and plants. However, the unprecedented use of tetracycline is of great concern owing to its low biodegradability, extensive usage, and adverse impacts on the environment and water quality. In this study, a sensitive spectrofluorometric method was proposed for the direct determination of tetracycline, based on biocompatible fluorescent carbon dots (CDs). The synthesis of CDs was performed by adopting a green hydrothermal procedure from carrot juice without requiring surface passivation or outflowing any environmentally hazardous waste. X-ray diffraction analysis and transmission electron microscopy revealed amorphous spherical-shaped CDs that exhibited blue emission under blue illumination. The fabricated fluorescent probe directly detected tetracycline in the concentration range of 4.00 × 10 to 1.55 × 10 mol L with an LOD of 1.33 × 10 mol L. The performance of the probe was assessed in a tap water sample, with recovery values between 80.70 and 103.60%. The method's greenness was evaluated using the Analytical Green metric approach (AGREE) and confirmed to be within the green range. The developed method is facile, rapid, cost-effective, and offers a wide linear range and satisfactory selectivity, making it potentially suitable for determining tetracycline in water applications.
抗生素四环素可以有效地用作人类、动物和植物细菌感染的预防药物。然而，由于四环素的生物降解性低、使用广泛以及对环境和水质的不利影响，其前所未有的使用引起了极大的关注。在本研究中，提出了一种基于生物相容性荧光碳点 (CDs) 的灵敏光谱荧光法直接测定四环素。通过采用从胡萝卜汁中进行绿色水热法，无需表面钝化或流出任何环境危害废物来合成 CDs。X 射线衍射分析和透射电子显微镜显示出无定形的球形 CDs，在蓝光照射下发出蓝色发射。所制备的荧光探针可直接在 4.00×10 到 1.55×10 mol L 的浓度范围内检测四环素，LOD 为 1.33×10 mol L。该探针在自来水样品中的性能进行了评估，回收率在 80.70%至 103.60%之间。采用分析绿色度量方法 (AGREE) 评估了该方法的绿色性，并证实其处于绿色范围内。该方法简便、快速、经济高效，具有较宽的线性范围和令人满意的选择性，使其有可能适用于水应用中四环素的测定。
