Department of Chemistry, University of Crete, Voutes University Campus, 70013, Heraklion, Greece.
Chembiochem. 2024 Nov 4;25(21):e202400514. doi: 10.1002/cbic.202400514. Epub 2024 Sep 3.
The primary objective of this work is to develop a sustainable biocatalytic transesterification process for low-grade oils, aligning with EU green technology requirements for the shift to second generation biodiesel. Thus, we investigated the immobilization and subsequent application of the lipase Biolipasa-R on transesterification processes to produce fatty acid methyl esters (FAMEs) from both a sunflower oil and an acid oil which is a bioproduct of the biodiesel industry. The lipase was immobilized on biomaterials, such as diatomaceous earth, with a yield of 60 %, and commercial carriers such as methacrylic resins with a yield of 100 %. The enzyme demonstrated superior activity when immobilized on diatomaceous earth, particularly in reactions involving the acid oil, outperforming the benchmark enzyme Novozym® 435 (95.1 % and 35 % conversion respectively). This work highlights the potential of Biolipasa-R as a cost-effective and efficient biocatalyst for biodiesel production and emphasizes the environmental benefits of utilizing industrial byproducts and eco-friendly immobilization techniques. The findings suggest that Biolipasa-R is a promising candidate for industrial applications in biodiesel production, offering a sustainable solution for waste management and energy generation.
本工作的主要目标是开发一种可持续的生物催化酯交换工艺，用于转化低等级油脂，以满足欧盟绿色技术要求，推动向第二代生物柴油的转变。因此，我们研究了脂肪酶 Biolipasa-R 的固定化及其在酯交换过程中的后续应用，以从向日葵油和一种酸油（生物柴油工业的生物产物）中生产脂肪酸甲酯（FAME）。脂肪酶通过固定在硅藻土等生物材料上（产率为 60%）和甲基丙烯酸树脂等商业载体上（产率为 100%）实现了固定化。与商业酶 Novozym® 435 相比，当固定在硅藻土上时，该酶在涉及酸油的反应中表现出更高的活性（转化率分别为 95.1%和 35%）。这项工作突出了 Biolipasa-R 作为生物柴油生产中具有成本效益和高效的生物催化剂的潜力，并强调了利用工业副产品和环保型固定化技术的环境效益。研究结果表明，Biolipasa-R 是生物柴油生产中具有应用前景的候选酶，为废物管理和能源生产提供了可持续的解决方案。
