Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, ZC 91501-970, Brazil.
Department of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, ZC 28049, Madrid, Spain.
Appl Biochem Biotechnol. 2018 Nov;186(3):576-589. doi: 10.1007/s12010-018-2763-x. Epub 2018 Apr 21.
This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was performed in a mixture design of three factors to obtain the ideal mixture of lipases according to the composition of fatty acids present in each oil, and the main reaction variables were optimized. After 18 h of reaction, ultrasound provided a biodiesel yield of about 90% when using soybean oil and 70% using the waste oil. The results showed that ultrasound technology, in combination with the application of enzyme mixtures, known as combi-lipases, and the use of waste oil, could be a promising route to reduce the overall process costs of enzymatic production of biodiesel.
本工作描述了使用超声系统在油脂的酶促转酯化中使用复合脂肪酶作为生物催化剂。通过评估废油和新鲜大豆油的单独使用，并使用固定化脂肪酶 CALB、TLL 和 RML 作为生物催化剂，进行了三因素的混合物设计，以根据每种油中存在的脂肪酸的组成获得理想的脂肪酶混合物，并对主要反应变量进行了优化。在 18 小时的反应后，使用大豆油时超声提供了约 90%的生物柴油产率，而使用废油时则提供了 70%的生物柴油产率。结果表明，超声技术与酶混合物（称为复合脂肪酶）的应用以及废油的使用相结合，可能是降低酶法生产生物柴油整体工艺成本的有前途的途径。
