Neamatollahi Ali Nour, Tarashi Samira, Ebrahimzadeh Nayereh, Vaziri Farzam, Zaheri Birgani Mohammad Ali, Aghasadeghi Mohammadreza, Fateh Abolfazl, Davar Siadat Seyed, Bouzari Saeid
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
Iran J Microbiol. 2024 Jun;16(3):342-350. doi: 10.18502/ijm.v16i3.15766.
TB infection is one of the most challengeable epidemiological issues. Complex interactions between microbiota and TB infection have been demonstrated. Alteration in microbial population during TB infection may act as a useful biomarker. The present study examined the microbiota patterns of blood and sputum samples collected from Afghan immigrants and Iranian patients with active TB.
Sixty active pulmonary TB patients were enrolled in the study. Blood and sputum samples were collected. To detect phylum bacterial composition in the blood and sputum samples, bacterial 16S rRNA quantification by Real-Time qPCR was performed.
A significant decrease in Bacteroidetes in Iranian sputum and blood samples of Afghan immigrants and Iranian TB active subjects were seen. While, sputum samples of Afghan immigrants showed no significant differences in Bacteroidetes abundance among TB active and control. Firmicutes were also presented no significant difference between sputum samples of the two races. Actinobacteria showed a significant increase in Iranian and Afghan sputum samples while this phylum showed no significant abundance in Iranian and Afghan TB positive blood samples. Proteobacteria also showed an increase in sputum and blood samples of the two races.
An imbalance in Bacteroidetes and Firmicutes abundance may cause an alteration in the microbiota composition, resulting in dysregulated immune responses and resulting in the augmentation of opportunistic pathogens during TB infection, notably Proteobacteria and Actinobacteria. Evaluation of human microbiota under different conditions of TB infection can be critical to a deeper understanding of the disease control.
结核病感染是最具挑战性的流行病学问题之一。微生物群与结核病感染之间复杂的相互作用已得到证实。结核病感染期间微生物种群的改变可能是一种有用的生物标志物。本研究检测了从阿富汗移民和患有活动性结核病的伊朗患者采集的血液和痰液样本中的微生物群模式。
60例活动性肺结核患者纳入本研究。采集血液和痰液样本。为检测血液和痰液样本中的细菌门类组成，采用实时定量聚合酶链反应对细菌16S rRNA进行定量分析。
在阿富汗移民和伊朗结核病活动期受试者的伊朗痰液和血液样本中，拟杆菌门显著减少。而阿富汗移民的痰液样本在结核病活动期和对照组之间拟杆菌门丰度无显著差异。两个种族的痰液样本中厚壁菌门也无显著差异。放线菌门在伊朗和阿富汗的痰液样本中显著增加，而在伊朗和阿富汗结核病阳性血液样本中该门类丰度无显著差异。变形菌门在两个种族的痰液和血液样本中也有所增加。
拟杆菌门和厚壁菌门丰度失衡可能导致微生物群组成改变，导致免疫反应失调，并导致结核病感染期间机会性病原体增加，尤其是变形菌门和放线菌门。在不同结核病感染条件下评估人类微生物群对于深入了解疾病控制可能至关重要。
