Department of Clinical Laboratory, Peking Union Medical College Hospital, No.1 Shuaifuyuan Wangfujing Dongcheng District, Beijing, 100730, China.
Department of Clinical Laboratory, Air Force Medical Center, 30 Fucheng Road, Haidian District, Beijing, 100142, China.
Tuberculosis (Edinb). 2022 Sep;136:102231. doi: 10.1016/j.tube.2022.102231. Epub 2022 Jul 21.
To investigate the characteristic of nasopharyngeal microbiota at different states of Mycobacterium tuberculosis (MTB) infection.
Participants were recruited from a chest hospital and were divided into three groups: the active tuberculosis (ATB) group, the latent TB infection (LTBI) group and the healthy control (HC) group. Nasopharyngeal microbiota was analyzed by 16S rRNA sequencing and clinical laboratory test results of ATB patients were collected and statistically analyzed.
Eleven ATB patients, 19 LTBI individuals and 18 healthy controls were included. Compared with LTBI group, Proteobacteria (P=0.04) and Gammaproteobacteria (P=0.01) increased in the ATB group. Compared with HC group, Pseudomonadales (P=0.03) and Moraxellaceae (P=0.04) increased, while Bacillales (P=0.04) and Lachnospiraceae (P=0.03) decreased in ATB group. Furthermore, Staphylococcus and Corynebacterium accounted for 70-80% in HC and LTBI groups. While in ATB group, they were less than 40%. Moreover, relative abundance of Corynebacterium, Corynebacteriaceae and Mycobacteriales was positively correlated with serum adenosine deaminase while negatively correlated with albumin, hemoglobin, and platelet counts in ATB patients.
The composition of nasopharyngeal microbiota changed significantly after MTB infection. The correlations between Corynebacterium and nutritional status (hemoglobin and albumin), immune-related molecules (adenosine deaminase) and inflammation-related indicators (platelet) in ATB patients deserve further exploration.
探讨结核分枝杆菌（MTB）感染不同状态时鼻咽部微生物群的特征。
从胸科医院招募参与者，分为活动性肺结核（ATB）组、潜伏性结核感染（LTBI）组和健康对照组（HC）。通过 16S rRNA 测序分析鼻咽部微生物群，并收集 ATB 患者的临床实验室检测结果进行统计分析。
纳入 11 例 ATB 患者、19 例 LTBI 个体和 18 例健康对照者。与 LTBI 组相比，ATB 组中变形菌门（P=0.04）和γ变形菌纲（P=0.01）增加。与 HC 组相比，ATB 组中假单胞菌目（P=0.03）和莫拉菌科（P=0.04）增加，而芽孢杆菌目（P=0.04）和lachnospiraceae 科（P=0.03）减少。此外，在 HC 和 LTBI 组中，葡萄球菌属和棒状杆菌属占 70-80%。而在 ATB 组中，它们的含量低于 40%。此外，ATB 患者中棒状杆菌属、棒状杆菌科和分枝杆菌目相对丰度与血清腺苷脱氨酶呈正相关，与白蛋白、血红蛋白和血小板计数呈负相关。
MTB 感染后鼻咽部微生物群组成发生明显变化。ATB 患者中棒状杆菌与营养状况（血红蛋白和白蛋白）、免疫相关分子（腺苷脱氨酶）和炎症相关指标（血小板）之间的相关性值得进一步探讨。
