Oral inoculation of , DNA quantification and histopathological evaluation of gingival, heart and kidney tissue samples in rats.

BACKGROUND AND OBJECTIVES

is known as common pathogen for endodontic infections and cause secondary and refractory pulp periapical periodontitis. The bacteria can opportunistically colonize periodontal pockets and presents a possibility of infection developing in other organs. This research will investigate the dissemination of from the gingival tissue to the heart and kidney.

MATERIALS AND METHODS

Three groups were formed, consisting of twelve male Sprague Dawley rats: a control group designated as 0-day, and experimental groups labeled as 7-days and 14-days. Periodontitis induced by concurrent infection with sterile wire 0.2 mm insertion and inoculation is performed into the gingival sulcus located between the maxillary right 1 and 2 molar teeth area. After euthanasia, tissue samples around the maxillary gingiva, maxillary jaw samples, kidney and heart tissues were obtained for quantitative Real-Time PCR assay and histopathological analysis.

RESULTS

Results showed at 7-days, there was an upregulation of gene expression in the gingiva, heart, and kidney samples as well as infiltration of the inflammatory cells at 7-days post induction, which consequently decreased at 14-days.

CONCLUSION

Thus, the study suggests dissemination of s from gingival tissue to the heart, kidney which could be probable link between periodontal disease, heart, and kidney disease.