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一种检测和测定C1的简单方法。

A simple method for the detection and assay of C1.

作者信息

Folkerd E J, Hughes-Jones N C

出版信息

J Immunol Methods. 1979;31(1-2):187-9. doi: 10.1016/0022-1759(79)90300-4.

DOI:10.1016/0022-1759(79)90300-4
PMID:390056
Abstract

A simple method is described for the production of human serum deficient in the complement component C1. This C1-deficient serum can be used for the assay of C1. If the amount of C1 is expressed in terms of its subcomponent C1q, the method can detect C1 when the C1q subcomponent content is only 10 pg.

摘要

本文描述了一种制备缺乏补体成分C1的人血清的简单方法。这种缺乏C1的血清可用于C1的测定。如果以其亚成分C1q来表示C1的量,当C1q亚成分含量仅为10 pg时,该方法就能检测到C1。

相似文献

1
A simple method for the detection and assay of C1.一种检测和测定C1的简单方法。
J Immunol Methods. 1979;31(1-2):187-9. doi: 10.1016/0022-1759(79)90300-4.
2
A simple one-step procedure for preparation of C1-deficient human serum.一种制备C1缺陷型人血清的简单一步法。
J Immunol Methods. 1984 Apr 27;69(2):277-80. doi: 10.1016/0022-1759(84)90325-9.
3
The first component of complement from the bullfrog, Rana catesbeiana: functional properties of C1- and isolation of subcomponent C1q.牛蛙(美国牛蛙)补体的首个成分:C1的功能特性及亚成分C1q的分离
J Immunol. 1980 Mar;124(3):1418-25.
4
Activation of C1 by soluble IgG aggregates as detected by a novel one-step hemolytic assay that specifically measures the proenzyme form of C1s.通过一种新型一步溶血试验检测可溶性IgG聚集体对C1的激活,该试验专门测量C1s的酶原形式。
J Immunol. 1983 Oct;131(4):1924-9.
5
Activation of the first component of complement, C1, by a monoclonal antibody recognizing the C chain of C1q.一种识别C1q C链的单克隆抗体激活补体的第一成分C1。
J Immunol. 1984 Feb;132(2):804-8.
6
C1q: isolation from human serum in high yield by affinity chromatography and development of a highly sensitive hemolytic assay.C1q:通过亲和色谱法从人血清中高产率分离及高灵敏度溶血测定法的开发
J Immunol. 1979 May;122(5):2103-11.
7
Inhibition of C1-mediated immune hemolysis by monomeric and dimeric peptides from the second constant domain of human immunoglobulin G.人免疫球蛋白G第二恒定结构域的单体和二聚体肽对C1介导的免疫溶血的抑制作用
J Immunol. 1981 Dec;127(6):2555-60.
8
[Stepwise dissociation of subcomponents of C1, the first component of human complement, upon activation on an affinity sorbent].[人补体第一成分C1在亲和吸附剂上激活后各亚组分的逐步解离]
Biokhimiia. 1989 Oct;54(10):1745-51.
9
Prevention of immune precipitation by purified classical pathway complement components.利用纯化的经典途径补体成分预防免疫沉淀。
Clin Exp Immunol. 1984 Nov;58(2):486-92.
10
Stoichiometry and sedimentation properties of the complex formed between the C1q and C1r2C1s2 subcomponents of the first component of complement.补体第一成分的C1q与C1r2C1s2亚成分之间形成的复合物的化学计量和沉降特性。
J Immunol. 1981 Dec;127(6):2447-52.

引用本文的文献

1
The relationship between the binding ability and the rate of activation of the complement component C1.补体成分C1的结合能力与激活速率之间的关系。
Immunology. 1980 Sep;41(1):179-85.