利用纯化的经典途径补体成分预防免疫沉淀。
Prevention of immune precipitation by purified classical pathway complement components.
作者信息
Naama J K, Hamilton A O, Yeung-Laiwah A C, Whaley K
出版信息
Clin Exp Immunol. 1984 Nov;58(2):486-92.
The role of the classical pathway of complement in the prevention of immune precipitation has been investigated using purified complement components and immune complexes (IC) consisting of rabbit anti-BSA and BSA. C1 reduced the rate of immune precipitation. As C1q, EDTA treated C1 or C1-inhibitor treated C1 were unable to retard the precipitation of IC, it was concluded that the intact C1 molecule was required for this function. Use of phenylmethylsulphonyl fluoride and benzamidine showed that the enzymatic site on C1 was not required for this activity. C4 and C2 did not affect immune precipitation significantly when C1 was present at the concentrations present in serum. When C3 was added to C1, C4 and C2 precipitation of IC did not occur. These data demonstrate that classical pathway activation alone is sufficient for the prevention of immune precipitation.
利用纯化的补体成分以及由兔抗牛血清白蛋白（BSA）和牛血清白蛋白组成的免疫复合物（IC），对补体经典途径在预防免疫沉淀中的作用进行了研究。C1降低了免疫沉淀的速率。由于C1q、经乙二胺四乙酸（EDTA）处理的C1或经C1抑制剂处理的C1均无法延缓IC的沉淀，因此得出结论，该功能需要完整的C1分子。使用苯甲基磺酰氟和苯甲脒表明，C1上的酶活性位点对此活性并非必需。当血清中存在的C1处于相应浓度时，C4和C2对免疫沉淀没有显著影响。当将C3添加到C1、C4和C2中时，IC没有发生沉淀。这些数据表明，仅经典途径的激活就足以预防免疫沉淀。
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