Varmira Kambiz, Kahrizi Danial, Sanjari Azarm, Rashidi Khodabakhsh, Hosseinzadeh Leila, Amin Niloufar, Jalilian Fereshteh
Research Center of Oils and Fats, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Agricultural Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Iran J Pharm Res. 2024 Apr 8;23(1):e140666. doi: 10.5812/ijpr-140666. eCollection 2024 Jan-Dec.
This study assessed the acute and sub-chronic toxicity of Camelina oil, a well-known oil rich in polyunsaturated fatty acids that enhance cellular immunity and human health, in Wistar rats. Wistar rats, 5 per sex per group, were randomly assigned to three groups for acute (14 days) toxicity studies and five groups for sub-chronic (90 days) toxicity studies. In the acute study, Camelina sativa oil was administered orally at a single dose of 5000 mg/kg of body weight (BW). The positive control group received a single dose of 5 000 mg/kg BW Canola oil by gavage. In the sub-chronic study, Groups III-V received 250, 500, and 1 000 mg/kg BW of Camelina oil, while Groups I and II received ultra-pure water and Canola oil at a dose of 500 mg/kg BW, respectively. Throughout the experiment, clinical signs, mortality, and body weight were monitored. At the end of the sub-chronic study, hematological, biochemical, and histopathological investigations were conducted. Administration of Camelina oil and Canola had no significant effect on daily weight gain (P > 0.05) of the test rats. Serum calcium levels decreased while phosphorous levels increased in male rats treated with Camelina oil. Other hematological and biochemical parameters showed no significant differences or dose-response effects between control and seed oil groups in both sexes (P < 0.05). Moreover, in animal necropsy, there were no apparent lesions in the liver, heart, and kidney organs in any of the doses administered. In conclusion, the results suggest that oral administration of Camelina oil is unlikely to be toxic. Therefore, the possibility for the development of future human nutrition should be considered.
本研究评估了荠蓝油（一种富含多不饱和脂肪酸、能增强细胞免疫力并有益人体健康的知名油脂）对Wistar大鼠的急性和亚慢性毒性。每组按性别各5只Wistar大鼠，被随机分为三组进行急性（14天）毒性研究，五组进行亚慢性（90天）毒性研究。在急性研究中，以5000毫克/千克体重的单剂量经口给予荠蓝籽油。阳性对照组经灌胃给予5000毫克/千克体重的单剂量菜籽油。在亚慢性研究中，第三至五组分别给予250、500和1000毫克/千克体重的荠蓝油，而第一组和第二组分别给予超纯水和500毫克/千克体重的菜籽油。在整个实验过程中，监测临床体征、死亡率和体重。在亚慢性研究结束时，进行血液学、生物化学和组织病理学检查。给予荠蓝油和菜籽油对受试大鼠的日体重增加无显著影响（P>0.05）。用荠蓝油处理的雄性大鼠血清钙水平降低而磷水平升高。其他血液学和生物化学参数在对照组和种子油组的两性之间均未显示出显著差异或剂量反应效应（P<0.05）。此外，在动物尸检中，在所给予的任何剂量下，肝脏、心脏和肾脏器官均未出现明显病变。总之，结果表明经口给予荠蓝油不太可能有毒。因此，应考虑其在未来人类营养领域发展的可能性。
