神经纤维瘤蛋白2调节A549肺癌细胞系中哺乳动物Ste2样激酶1/2和大肿瘤抑制基因1的表达。
Neurofibromin 2 modulates Mammalian Ste2-like kinases1/2 and large tumor suppressor gene1 expression in A549 lung cancer cell line.
作者信息
Li Xu, Guo Zaiqiang, Yang Yang, Xiong Ying, Zhang Xia, Qiao Shubin, Wei Ke, Fang Jin, Ma Yonghuai
机构信息
Department of Geriatrics, Beijing Fengtai Hospital of Integrated Traditional Chinese and Modern Medicine Beijing 100072, China.
Department of Gastroenterology, Capital Medical University Electric Power Teaching Hospital Beijing 100073, China.
出版信息
Am J Transl Res. 2024 Jun 15;16(6):2571-2578. doi: 10.62347/TPCM6776. eCollection 2024.
AIM
To explore the impact of up- or down-regulation of Neurofibromin 2 (NF2) on the expression of downstream Hippo pathway genes, large tumor suppressor gene1 (LATS1), and phosphorylation of Mammalian Ste2-like kinases1/2 (MST1/2), in lung cancer cells.
METHODS
A549 lung cancer cells were used. The NF2 was down-regulated by si-RNA interference and upregulated by lentiviral vector mediated overexpression. The LATS1 and MST1/2 expressions were evaluated by real-time PCR and western blot.
RESULTS
Down-regulation of NF2 decreased LATS1 and MST1/2 level (P<0.05). Overexpression of NF2 increased LATS1 (P<0.05) and Mammalian Ste2-like kinases1 (MST1) (P<0.05), suggesting LATS1 and MST1 are modulated by NF2 in a lung cancer cell line.
CONCLUSIONS
NF2 mediates the downstream LATS1 and MST1/2 expressions in a lung cancer cell line.
目的
探讨上调或下调神经纤维瘤蛋白2（NF2）对肺癌细胞中河马通路下游基因大肿瘤抑制基因1（LATS1）的表达以及哺乳动物Ste2样激酶1/2（MST1/2）磷酸化的影响。
方法
采用A549肺癌细胞。通过小干扰RNA（si-RNA）干扰下调NF2，并通过慢病毒载体介导的过表达上调NF2。通过实时聚合酶链反应（PCR）和蛋白质免疫印迹法评估LATS1和MST1/2的表达。
结果
下调NF2降低了LATS1和MST1/2水平（P<0.05）。NF2过表达增加了LATS1（P<0.05）和哺乳动物Ste2样激酶1（MST1）（P<0.05），表明在肺癌细胞系中LATS1和MST1受NF2调节。
结论
在肺癌细胞系中，NF2介导下游LATS1和MST1/2的表达。
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