Rusco Giusy, Paventi Gianluca, Di Iorio Michele, Spano Mattia, Cerolini Silvia, Iaffaldano Nicolaia
Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, Italy.
Department of Chemistry and Technology of Drugs, Sapienza University of Rome, 00185 Rome, Italy.
Data Brief. 2024 Jun 11;55:110627. doi: 10.1016/j.dib.2024.110627. eCollection 2024 Aug.
Significant changes in the quality and metabolic profile of fresh turkey sperm as a result of both cryopreservation and reproductive age have already been individually confirmed in our previous studies. This new dataset adds a relevant piece to the tangled puzzle of changes in metabolite levels affecting cryopreserved turkey sperm quality, taking into consideration two different reproductive period ages. Fresh semen samples were collected at 44 and 56 weeks of age and exposed to the cryopreservation process. All fresh and frozen-thawed samples were subjected to analysis of mobility, viability and osmotic tolerance as parameters for evaluating the sperm quality, while NMR spectroscopy was used to assess the quantitative changes in water and lipid-soluble metabolites. Our results showed that the cryopreservation process significantly affected all of the measured qualitative parameters both at 44 and 56 weeks. Concerning the metabolic profile, a greater number of quantitative changes for both water and lipid-soluble components were found in frozen semen at 56 weeks than at 44 weeks of age. These data could contribute to identifying new strategies aimed at improving freezing procedures even as reproductive age increases.
在我们之前的研究中，已经分别证实了冷冻保存和生殖年龄对新鲜火鸡精子质量和代谢谱的显著影响。考虑到两个不同的生殖时期年龄，这个新数据集为影响冷冻保存火鸡精子质量的代谢物水平变化这一错综复杂的难题增添了相关内容。在44周龄和56周龄时采集新鲜精液样本，并使其经历冷冻保存过程。所有新鲜和冻融样本都进行了运动性、活力和渗透耐受性分析，作为评估精子质量的参数，同时使用核磁共振光谱法评估水溶性和脂溶性代谢物的定量变化。我们的结果表明，冷冻保存过程在44周龄和56周龄时均显著影响所有测量的质量参数。关于代谢谱，56周龄冷冻精液中水溶性和脂溶性成分的定量变化数量比44周龄时更多。这些数据有助于确定即使随着生殖年龄增加仍旨在改善冷冻程序的新策略。
