Department of Animal, Plant and Environmental Science, University of Molise, Campobasso, Italy.
Theriogenology. 2011 Sep 15;76(5):794-801. doi: 10.1016/j.theriogenology.2011.04.012. Epub 2011 Jun 12.
This study was designed to identify the best pellet cryopreservation procedure for the cryosurvival of turkey semen among 192 different treatments established by variations and permutations of seven conditions used in the freezing/thawing process. These conditions were: diluent (IGGKPh, SPh or Tselutin); dilution rate (1:3 vs. 1:4); cooling time (45 vs. 60 min); dimethylacetamide (DMA) concentration as cryoprotectant (6 vs. 8%); equilibration time in DMA (1 vs. 5 min); semen drop volume (50 vs. 80 μL) and thawing temperature (60 vs. 75 °C). Through principal components analysis (PCA), post-thaw sperm quality data (mobility, viability and membrane functional integrity) were reduced to a single output variable (Sperm Quality) indicating overall post-thaw semen quality. All treatments induced a significant reduction in semen quality after warming (P < 0.01), though one set of seven conditions, or treatment, was identified by PCA to generate the highest Sperm Quality score and a further five treatments yielded a score not significantly different (P > 0.05) from this best score. Although still not fulfilling the requirements for commercial application, our findings serve to identify the critical steps in turkey sperm cryopreservation that need to be assessed in future studies.
本研究旨在确定在冷冻/解冻过程中使用的七种条件的变化和排列建立的 192 种不同处理方法中，哪种方法最适合火鸡精液颗粒的冷冻保存。这些条件是：稀释剂（IGGKPh、SPh 或 Tselutin）；稀释率（1:3 与 1:4）；冷却时间（45 分钟与 60 分钟）；二甲基乙酰胺（DMA）作为冷冻保护剂的浓度（6%与 8%）；DMA 平衡时间（1 分钟与 5 分钟）；精液滴体积（50 μL 与 80 μL）和解冻温度（60°C 与 75°C）。通过主成分分析（PCA），将解冻后精子质量数据（活力、活力和膜功能完整性）简化为单个输出变量（精子质量），表明整体解冻后精液质量。所有处理方法在加热后都会导致精液质量显著下降（P < 0.01），尽管通过 PCA 确定了一组七种条件或处理方法可以产生最高的精子质量评分，还有另外五种处理方法的评分与最佳评分没有显著差异（P > 0.05）。尽管仍然不符合商业应用的要求，但我们的研究结果有助于确定火鸡精子冷冻保存中需要在未来研究中评估的关键步骤。
