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基于循环circRNA谱改变筛选复发/难治性急性髓系白血病的预后基因特征

Screening of a Prognostic Gene Signature for Relapsed/Refractory Acute Myeloid Leukemia Based on Altered Circulating CircRNA Profiles.

作者信息

Guo Honggang, Cui Yabin, Bai Yanliang, Yan Fan, Zhang Wenhui, Chen Yuqing, Shi Mingyue

机构信息

Department of Hematology, Zhengzhou University People's Hospital and Henan Provincial People's Hospital, Zhengzhou, People's Republic of China.

Department of Hematology, Henan University People's Hospital, Zhengzhou, People's Republic of China.

出版信息

Int J Gen Med. 2024 Jul 8;17:2967-2979. doi: 10.2147/IJGM.S466364. eCollection 2024.

DOI:10.2147/IJGM.S466364
PMID:39006913
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11244134/
Abstract

BACKGROUND

Relapsed/refractory acute myeloid leukemia (R/R-AML) has dismal prognosis due to chemotherapy resistance. Circular RNAs (circRNAs) have shown emerging roles in chemotherapy resistance in various cancers including hematologic malignancies. However, the potential roles of circRNAs in AML progression and drug resistance remain largely undetermined.

METHODS

In this study, circulating circRNAs expression profiles were analyzed among R/R-AML, AML and healthy controls (HC) using a human circRNA Array. Bioinformatic analysis was carried out to explore the differentially expressed circRNAs (DE-circRNAs). GO, KEGG pathway analysis, along with circRNA-miRNA-mRNA network analysis, were conducted to identify the potential biological pathways involved in R/R-AML. Finally, the UALCAN database was used to assess the prognosis of different target DE-circRNAs-related mRNAs.

RESULTS

Forty-eight DE-circRNAs were upregulated, whereas twenty-seven DE-circRNAs were downregulated in R/R-AML samples. Up-regulated DE-circRNAs in R/R-AML samples were mainly enrichment in the biological processes and pathways of cell migration, microRNAs in cancers, Rap1 and Ras signaling pathways. Six DE-circRNAs were randomly selected to further explore their relationships with R/R-AML. GO and KEGG pathway analyses of the six candidate DE-circRNAs-related target mRNAs were mainly involved in the regulation of signal transduction and Ras signaling pathway. By overlapping our RNA-sequencing results of differentially expressed genes (DEGs) in R/R-AML samples with the candidate DE-circRNAs-predicted target mRNAs, we identified sixty-eight overlapping targeted mRNAs. Using UALCAN database analysis, we identified that AML patients with six upregulated DE-circRNA-related genes (ECE1, PI4K2A, SLC9A6, CCND3, PPP1R16B, and TRIM32) and one downregulated gene DE-circRNA-related genes (ARHGAP10) might have a poor prognosis.

CONCLUSION

This study revealed the overall alterations of circRNAs in R/R-AML. DE-circRNAs and their related genes might be used as potential early, sensitive and stable biomarkers for AML diagnosis, R/R-AML monitoring, and even as novel treatment targets for R/R-AML.

摘要

背景

复发/难治性急性髓系白血病（R/R-AML）由于化疗耐药，预后不佳。环状RNA（circRNA）在包括血液系统恶性肿瘤在内的多种癌症的化疗耐药中发挥着越来越重要的作用。然而，circRNA在AML进展和耐药中的潜在作用仍 largely未确定。

方法

在本研究中，使用人circRNA阵列分析了R/R-AML、AML和健康对照（HC）之间的循环circRNA表达谱。进行生物信息学分析以探索差异表达的circRNA（DE-circRNA）。进行了GO、KEGG通路分析以及circRNA-miRNA-mRNA网络分析，以确定R/R-AML中潜在的生物学通路。最后，使用UALCAN数据库评估不同靶标DE-circRNA相关mRNA的预后。

结果

在R/R-AML样本中，48个DE-circRNA上调，而27个DE-circRNA下调。R/R-AML样本中上调的DE-circRNA主要富集于细胞迁移、癌症中的微小RNA、Rap1和Ras信号通路的生物学过程和通路。随机选择6个DE-circRNA以进一步探索它们与R/R-AML的关系。对6个候选DE-circRNA相关靶标mRNA的GO和KEGG通路分析主要涉及信号转导和Ras信号通路的调节。通过将我们在R/R-AML样本中差异表达基因（DEG）的RNA测序结果与候选DE-circRNA预测的靶标mRNA进行重叠，我们鉴定出68个重叠的靶标mRNA。使用UALCAN数据库分析，我们确定，具有6个上调的DE-circRNA相关基因（ECE1、PI4K2A、SLC9A6、CCND3、PPP1R16B和TRIM32）和1个下调的基因DE-circRNA相关基因（ARHGAP10）的AML患者可能预后不良。

结论

本研究揭示了R/R-AML中circRNA的总体变化。DE-circRNA及其相关基因可能用作AML诊断、R/R-AML监测的潜在早期、敏感和稳定生物标志物，甚至可作为R/R-AML的新型治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa19/11244134/444f2ffc059e/IJGM-17-2967-g0007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa19/11244134/06c73daa5baa/IJGM-17-2967-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa19/11244134/f33253bb270b/IJGM-17-2967-g0002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa19/11244134/444f2ffc059e/IJGM-17-2967-g0007.jpg

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本文引用的文献

1
circSLC25A13 acts as a ceRNA to regulate AML progression via miR-616-3p/ADCY2 axis.环状 RNA SLC25A13 通过 miR-616-3p/ADCY2 轴作为 ceRNA 调控 AML 进展。
Mol Carcinog. 2023 Oct;62(10):1546-1562. doi: 10.1002/mc.23598. Epub 2023 Jul 26.
2
Advances in the protein‑encoding functions of circular RNAs associated with cancer (Review).环状 RNA 与癌症相关的蛋白编码功能的研究进展（综述）。
Oncol Rep. 2023 Aug;50(2). doi: 10.3892/or.2023.8597. Epub 2023 Jul 14.
3
Gene SH3BGRL3 regulates acute myeloid leukemia progression through circRNA_0010984 based on competitive endogenous RNA mechanism.
基因SH3BGRL3基于竞争性内源RNA机制通过circRNA_0010984调控急性髓系白血病进展。
Front Cell Dev Biol. 2023 Jun 12;11:1173491. doi: 10.3389/fcell.2023.1173491. eCollection 2023.
4
EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway.EIF4A3诱导的Circ_0001187通过促进METTL3的泛素-蛋白酶体降解以及降低由miR-499a-5p/RNF113A途径介导的m6A修饰水平来促进急性髓系白血病的抑制。
Biomark Res. 2023 Jun 6;11(1):59. doi: 10.1186/s40364-023-00495-4.
5
Verification of prognostic expression biomarkers is improved by examining enriched leukemic blasts rather than mononuclear cells from acute myeloid leukemia patients.通过检测富集的白血病原始细胞而非急性髓系白血病患者的单核细胞，可改善预后表达生物标志物的验证。
Biomark Res. 2023 Mar 16;11(1):31. doi: 10.1186/s40364-023-00461-0.
6
New drugs before, during, and after hematopoietic stem cell transplantation for patients with acute myeloid leukemia.造血干细胞移植前后新药物治疗急性髓细胞白血病患者。
Haematologica. 2023 Feb 1;108(2):321-341. doi: 10.3324/haematol.2022.280798.
7
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J Exp Clin Cancer Res. 2022 Sep 7;41(1):267. doi: 10.1186/s13046-022-02482-3.
8
CircRNA-CREIT inhibits stress granule assembly and overcomes doxorubicin resistance in TNBC by destabilizing PKR.环状 RNA-CREIT 通过使 PKR 不稳定来抑制应激颗粒组装并克服 TNBC 对多柔比星的耐药性。
J Hematol Oncol. 2022 Aug 29;15(1):122. doi: 10.1186/s13045-022-01345-w.
9
Up-Regulation of TRIM32 Associated With the Poor Prognosis of Acute Myeloid Leukemia by Integrated Bioinformatics Analysis With External Validation.通过综合生物信息学分析及外部验证揭示TRIM32上调与急性髓系白血病不良预后相关
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Ann Hematol. 2022 Aug;101(8):1703-1710. doi: 10.1007/s00277-022-04862-3. Epub 2022 May 20.