Fujian Institute of Hematology, Fujian Medical Center of Hematology, Clinical Research Center for Hematological Malignancies of Fujian Province, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, Fujian, 350001, China.
Medical Intensive Care Unit, Fujian Provincial Hospital, Fuzhou, Fujian, 35001, China.
Biochem Biophys Res Commun. 2022 Apr 23;601:137-145. doi: 10.1016/j.bbrc.2022.02.087. Epub 2022 Feb 23.
Acute myeloid leukemia (AML) is the most common acute leukemia in adults, associated with poor prognosis and easy relapse of disease. Circular RNAs (circRNAs) were detected to be mA modified and the role of mA circRNAs has been reported in other diseases including cancers, however, their role has not been elucidated in AML yet. In the present study, we aimed to investigate the expression profiling of mA circRNAs in AML. We performed mA circRNAs microarray analysis to identify differentially expressed mA circRNAs in bone marrow samples from AML patients and healthy individuals (control). Furthermore, bioinformatics analysis predicted the potential functions and relevant pathways that may be associated with the mA circRNAs. The circRNA mA methylation levels were found to be positively associated with the circRNAs expression, suggesting circRNA mA modification could contribute to circRNA regulation in AML. Further analysis demonstrated that circRNA mA modification might influence the circRNA-miRNA-mRNA co-expression network that may contribute to the circRNA regulatory network in AML. Our findings provide evidence of the differential expression profile of mA circRNAs in AML, and circRNA mA modification may contribute to circRNA regulatory function in AML.
急性髓系白血病（AML）是成人中最常见的急性白血病，预后不良，疾病易复发。环状 RNA（circRNA）被检测到发生 mA 修饰，mA circRNAs 的作用已在包括癌症在内的其他疾病中得到报道，但其在 AML 中的作用尚未阐明。在本研究中，我们旨在研究 AML 中 mA circRNAs 的表达谱。我们进行了 mA circRNAs 微阵列分析，以鉴定 AML 患者和健康个体（对照）骨髓样本中差异表达的 mA circRNAs。此外，生物信息学分析预测了可能与 mA circRNAs 相关的潜在功能和相关途径。circRNA mA 甲基化水平与 circRNAs 的表达呈正相关，提示 circRNA mA 修饰可能有助于 AML 中 circRNA 的调控。进一步的分析表明，circRNA mA 修饰可能影响 circRNA-miRNA-mRNA 共表达网络，这可能有助于 AML 中的 circRNA 调控网络。我们的研究结果为 AML 中 mA circRNAs 的差异表达谱提供了证据，并且 circRNA mA 修饰可能有助于 AML 中 circRNA 的调控功能。
