Albertini S, Friederich U, Gröschel-Stewart U, Zimmermann F K, Würgler F E
Mutat Res. 1985 Oct;144(2):67-71. doi: 10.1016/0165-7992(85)90003-x.
Phenobarbital (PB) specifically induces mitotic chromosomal malsegregation in the diploid Saccharomyces cerevisiae strain D61.M but no other genetic events such as mitotic recombination or point mutations. In accordance with the hypothesis that PB exerts its genotoxic activity by an interaction with tubulin, it stimulates the GTP-promoted assembly of porcine brain tubulin in vitro. This process is reversible thus excluding an unspecific denaturation of the tubulin protein by PB.
苯巴比妥（PB）能特异性地诱导二倍体酿酒酵母菌株D61.M出现有丝分裂染色体错误分离，但不会引发其他遗传事件，如有丝分裂重组或点突变。根据PB通过与微管蛋白相互作用发挥其遗传毒性活性的假说，它在体外能刺激猪脑微管蛋白的GTP促进组装。这个过程是可逆的，因此排除了PB对微管蛋白的非特异性变性作用。
