Department of Medicine, Graduate School, Wonkwang University, Iksan, Republic of Korea.
Sarcopenia Total Solution Center, Wonkwang University, Iksan, Republic of Korea.
Zebrafish. 2024 Oct;21(5):320-328. doi: 10.1089/zeb.2024.0128. Epub 2024 Jul 15.
Global warming and extreme weather events pose a significant threat to global biodiversity, with rising water temperatures exerting a profound influence on fish conservation and fishery development. In this study, we used zebrafish as a model organism to explore the impact of a heat acclimation period on their survival rates. The results demonstrated that a 2-month heat acclimation period almost completely mitigated heat stress-induced mortality in zebrafish. Subsequent analysis of the surviving zebrafish revealed a predominance of hepatic mitochondria in a fission state. Remarkably, a short-term fasting regimen, which induced hepatic mitochondrial fission, mirrored the outcomes of the protective effect of heat acclimation and augmented animal survival under heat stress. Conversely, treatment with a mitochondrial fission inhibitor within the fasting group attenuated the elevated survival rate. Furthermore, zebrafish embryos subjected to brief heat acclimation also exhibited increased heat resistance, a trait diminished by a chemical intervention inhibiting mitochondrial fission. This suggests a shared mechanism for heat resistance between embryos and adult zebrafish. These findings underscore the potential use of inducing mitochondrial fission to enhance heat resistance in zebrafish, offering promise for fish biodiversity conservation in the face of global warming.
全球变暖和极端天气事件对全球生物多样性构成重大威胁，水温升高对鱼类保护和渔业发展产生深远影响。在这项研究中，我们使用斑马鱼作为模型生物，探讨了热适应期对其存活率的影响。结果表明，为期 2 个月的热适应期几乎完全缓解了热应激引起的斑马鱼死亡。对幸存斑马鱼的后续分析显示，肝脏线粒体以分裂状态为主。值得注意的是，短期禁食方案诱导肝脏线粒体分裂，模拟了热适应的保护效果，并提高了动物在热应激下的存活率。相反，在禁食组中使用线粒体分裂抑制剂处理会降低存活率的提高。此外，短暂热适应的斑马鱼胚胎也表现出更高的耐热性，而用化学干预抑制线粒体分裂会削弱这种特性。这表明胚胎和成年斑马鱼之间存在耐热性的共同机制。这些发现强调了诱导线粒体分裂以提高斑马鱼耐热性的潜力，为应对全球变暖保护鱼类生物多样性提供了可能。
