Instituto de Neurociencias, Universidad Miguel Hernández - Consejo Superior de Investigaciones Científicas, Alicante, Spain.
Adv Neurobiol. 2024;38:111-129. doi: 10.1007/978-3-031-62983-9_7.
Memory traces for behavioral experiences, such as fear conditioning or taste aversion, are believed to be stored through biophysical and molecular changes in distributed neuronal ensembles across various brain regions. These ensembles are known as engrams, and the cells that constitute them are referred to as engram cells. Recent advancements in techniques for labeling and manipulating neural activity have facilitated the study of engram cells throughout different memory phases, including acquisition, allocation, long-term storage, retrieval, and erasure. In this chapter, we will explore the application of next-generation sequencing methods to engram research, shedding new light on the contribution of transcriptional and epigenetic mechanisms to engram formation and stability.
行为经验的记忆痕迹，如恐惧条件反射或味觉厌恶，被认为是通过分布在不同大脑区域的神经元集合中的生物物理和分子变化来存储的。这些集合被称为记忆痕迹，构成它们的细胞被称为记忆痕迹细胞。用于标记和操纵神经活动的新技术的最新进展促进了对整个不同记忆阶段的记忆痕迹细胞的研究，包括获取、分配、长期储存、检索和擦除。在本章中，我们将探讨下一代测序方法在记忆痕迹研究中的应用，为转录和表观遗传机制对记忆痕迹形成和稳定性的贡献提供新的认识。
