Cerrahpasa Faculty of Medicine, Department of Medical Biochemistry, Istanbul University-Cerrahpasa, Istanbul, Turkey.
Cerrahpasa Faculty of Medicine, Department of Emergency Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey.
Mol Biol Rep. 2024 Jul 15;51(1):813. doi: 10.1007/s11033-024-09729-6.
We aimed to investigate the serum Nuclear Factor Kappa B (NF-κB) p105, NF-κB p65 and Inhibitor Kappa B Alpha (IκBα) levels in patients with mild/moderate Coronavirus Disease 2019 (COVID-19) and their association with the course of the disease.
Blood was drawn from 35 COVID-19 patients who applied to the Department of Emergency Medicine of Istanbul University-Cerrahpasa at the time of diagnosis and from 35 healthy individuals. The patients were evaluated to have mild/moderate degree of disease according to National Early Warning Score 2 (NEWS2) scoring and computed tomography (CT) findings. The markers were studied in the obtained serum samples, using enzyme-linked immunoassay (ELISA). Receiver Operating Characteristic (ROC) analysis was performed. Statistical significance was evaluated to be p < 0.05.
NF-κB p105 levels were significantly higher in the COVID-19 group compared to the control group. C-reactive protein (CRP), D-dimer, ferritin levels of the patients were significantly higher (p < 0.001) compared to the control group, while the lymphocyte count was found lower (p = 0.001). IκBα and NF-κB p65 levels are similar in both groups. Threshold value for NF-κB p105 was above 0.78 ng/mL, sensitivity was 71.4% and specificity was 97.1% (p < 0.05). NF-κB p105 levels at the time of diagnosis of the patients who required supplemental oxygen (O), were significantly higher (p < 0.01).
The rise in serum NF-κB p105 levels during the early stages of infection holds diagnostic value. Besides its relation with severity might have a prognostic feature to foresee the requirement for supplemental O that occurs during hospitalization.
本研究旨在探讨轻度/中度 2019 年冠状病毒病（COVID-19）患者血清核因子-κB（NF-κB）p105、NF-κB p65 和抑制物κB 阿尔法（IκBα）的水平及其与疾病进程的关系。
从伊斯坦布尔大学切拉帕萨急诊医学系就诊的 35 例 COVID-19 患者和 35 例健康个体的血液中提取血清。根据国家早期预警评分 2（NEWS2）评分和计算机断层扫描（CT）结果评估患者为轻度/中度疾病。使用酶联免疫吸附测定（ELISA）法研究获得的血清样本中的标志物。进行受试者工作特征（ROC）分析。p<0.05 为有统计学意义。
与对照组相比，COVID-19 组 NF-κB p105 水平显著升高。与对照组相比，患者的 C 反应蛋白（CRP）、D-二聚体和铁蛋白水平显著升高（p<0.001），而淋巴细胞计数较低（p=0.001）。两组的 IκBα 和 NF-κB p65 水平相似。NF-κB p105 的截断值为>0.78ng/ml，灵敏度为 71.4%，特异性为 97.1%（p<0.05）。需要补充氧气（O）的患者在诊断时的 NF-κB p105 水平显著升高（p<0.01）。
感染早期血清 NF-κB p105 水平的升高具有诊断价值。除了与严重程度的关系外，它还可能具有预测住院期间补充 O 需求的预后特征。
