电感耦合等离子体质谱法分析生物样本中的铁元素。
ICP-MS Analysis of Iron from Biological Samples.
机构信息
Nebraska Redox Biology Center, University of Nebraska-Lincoln, Lincoln, NE, USA.
出版信息
Methods Mol Biol. 2024;2839:31-41. doi: 10.1007/978-1-0716-4043-2_2.
Elemental analysis can provide trace concentrations of iron and other transition elements at nanomolar (μg/L) concentrations in whole bacterial and mammalian cells, subcellular compartments, biological fluids, and tissues. The best method of analysis is by far Inductively Coupled Plasma Mass Spectrometry (ICP-MS). I describe here a very general method for the sample preparation, instrument settings, method development, and analysis. The method can be extended to up to 20 common elements in biological samples.
元素分析可以提供痕量浓度的铁和其他过渡元素，在纳米摩尔（μg/L）浓度的全细菌和哺乳动物细胞、亚细胞区室、生物流体和组织中。目前最好的分析方法是电感耦合等离子体质谱法（ICP-MS）。我在这里描述了一种非常通用的样品制备方法、仪器设置、方法开发和分析方法。该方法可扩展到生物样品中多达 20 种常见元素。
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