Pluháček Tomáš, Lemr Karel, Ghosh Dipankar, Milde David, Novák Jiří, Havlíček Vladimír
Department of Analytical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacky University, 17. listopadu 12, 771 46, Olomouc, Czech Republic.
Institute of Microbiology, AS CR v.v.i., Videnska 1083, CZ 142 20, Prague 4, Czech Republic.
Mass Spectrom Rev. 2016 Jan-Feb;35(1):35-47. doi: 10.1002/mas.21461. Epub 2015 May 15.
Siderophores play important roles in microbial iron piracy, and are applied as infectious disease biomarkers and novel pharmaceutical drugs. Inductively coupled plasma and molecular mass spectrometry (ICP-MS) combined with high resolution separations allow characterization of siderophores in complex samples taking advantages of mass defect data filtering, tandem mass spectrometry, and iron-containing compound quantitation. The enrichment approaches used in siderophore analysis and current ICP-MS technologies are reviewed. The recent tools for fast dereplication of secondary metabolites and their databases are reported. This review on siderophores is concluded with their recent medical, biochemical, geochemical, and agricultural applications in mass spectrometry context.
铁载体在微生物铁掠夺中发挥着重要作用，并被用作传染病生物标志物和新型药物。电感耦合等离子体和质谱联用（ICP-MS）结合高分辨率分离技术，利用质量缺陷数据过滤、串联质谱和含铁化合物定量等方法，能够对复杂样品中的铁载体进行表征。本文综述了铁载体分析中使用的富集方法和当前的ICP-MS技术。还报道了用于次生代谢物快速去重复的最新工具及其数据库。本文在质谱背景下对铁载体的近期医学、生物化学、地球化学和农业应用进行了总结。
