Department of Molecular and Medical Genetics, Oregon Health & Sciences University, Portland, OR, USA.
Methods Mol Biol. 2024;2839:43-52. doi: 10.1007/978-1-0716-4043-2_3.
Characterizing the two- and three-dimensional distribution of trace metals in biological specimens is key to better understand their role in biological processes. Iron (Fe) is of particular interest in these trace metals due to its widespread role in maintaining cellular health and preventing disease. X-ray fluorescence microscopy (XFM) is emerging as the method of choice for investigators to interrogate the cellular and subcellular distribution of Fe. XFM utilizes the intrinsic X-ray fluorescence properties of each element to produce quantitative 2D and 3D distributions of trace metals within a sample. Herein, methods for sample preparation of cells and tissue for the determination of Fe distribution by XFM are described.
描述生物样本中痕量金属的二维和三维分布对于更好地理解它们在生物过程中的作用至关重要。在这些痕量金属中，铁（Fe）因其在维持细胞健康和预防疾病方面的广泛作用而特别受到关注。X 射线荧光显微镜（XFM）正成为研究人员研究 Fe 细胞和亚细胞分布的首选方法。XFM 利用每个元素的固有 X 射线荧光特性，在样品内产生痕量金属的定量 2D 和 3D 分布。本文描述了用于通过 XFM 测定 Fe 分布的细胞和组织样品制备方法。
