Department of Biological Sciences, University of Delaware, Newark, DE, USA.
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
Methods Mol Biol. 2024;2839:195-211. doi: 10.1007/978-1-0716-4043-2_10.
Recombinant expression and biogenesis of cytochrome c species is a simple and efficient method for the production of holocytochrome c species, thus presenting an avenue for the study of cytochrome c or the cytochrome c biogenesis pathways responsible for heme attachment. Here, we describe a method for recombinant E. coli production of holocytochrome c utilizing the System I (CcmABCDEFGH) bacterial cytochrome c biogenesis pathway, followed by analysis of cytochrome c species by cell lysis and heme stain.
细胞色素 c 物种的重组表达和生物发生是生产全细胞色素 c 物种的简单而有效的方法，因此为细胞色素 c 或负责血红素附着的细胞色素 c 生物发生途径的研究提供了途径。在这里，我们描述了一种利用系统 I（CcmABCDEFGH）细菌细胞色素 c 生物发生途径在重组大肠杆菌中生产全细胞色素 c 的方法，然后通过细胞裂解和血红素染色分析细胞色素 c 物种。
