The gene influences the multiple stresses tolerance by affecting cytochrome c in N8.

, an internationally recognized safe species of Gram-positive bacteria, produces a variety of antimicrobial peptides. In this study, we examined the gene of N8 and its influence on the bacterial stress response. We used the Cre-loxP system to knock out the gene from the N8Δ genome. The mutant strains N8Δ and ΔΔ showed higher nisin tolerance and nisin production than the wild-type strain N8, and also obtained tolerance for multiple stressors. The biological characteristics of the gene and its encoded protein in N8 were investigated. Deletion of the gene significantly reduced the content of cytochrome c, enhanced the tolerance of the strain to various environmental stresses, resulted in increased antimicrobial peptide nisin production and improved nisin resistance. Deletion of gene conferred resistance to multiple stressors, including lysozyme, lactic acid, and antibiotics, and demonstrated resistance against multiple bacteriostatic mechanisms. Our findings lay the foundation for further research into the physiological processes of stress resistance in lactic acid bacteria.