Theriogenology Laboratory, Southern Regional Station of ICAR-National Dairy Research Institute, Bengaluru, India.
Dairy Production Section, Southern Regional Station of ICAR-National Dairy Research Institute, Bengaluru, India.
Syst Biol Reprod Med. 2024 Dec;70(1):204-217. doi: 10.1080/19396368.2024.2370328. Epub 2024 Jul 15.
In the present study, a comparative global high-throughput proteomic analysis strategy was used to identify proteomic differences between estrus and diestrus stage of estrous cycle in dairy cows. Saliva was collected from cows during estrus and diestrus, and subjected to LC-MS/MS-based proteomic analysis. A total of 2842 proteins were detected in the saliva of cows, out of which, 2437 and 1428 non-redundant proteins were identified in estrous and diestrous saliva, respectively. Further, it was found that 1414 and 405 salivary proteins were specific to estrus and diestrus, respectively while 1023 proteins were common to both groups. Among the significantly dysregulated proteins, the expression of 56 proteins was down-regulated (abundance ratio <0.5) while 40 proteins were up-regulated (abundance ratio > 2) in estrous compared to diestrous saliva. The proteins, such as HSD17B12, INHBA, HSP70, ENO1, SRD5A1, MOS, AMH, ECE2, PDGFA, OPRK1, SYN1, CCNC, PLIN5, CETN1, AKR1C4, NMNAT1, CYP2E1, and CYP19A1 were detected only in the saliva samples derived from estrous cows. Considerable number of proteins detected in the saliva of estrous cows were found to be involved in metabolic pathway, PI3K-Akt signaling pathway, toll-like receptor signaling pathway, steroid biosynthesis pathway, insulin signaling pathway, calcium signaling pathway, estrogen signaling pathway, oxytocin signaling pathway, TGF-β signaling pathway and oocyte meiosis. On the other hand, proteins detected in saliva of diestrous cows were involved mainly in metabolic pathway. Collectively, these data provide preliminary evidence of a potential difference in salivary proteins at different stages of estrous cycle in dairy cows.
在本研究中，采用比较全面的高通量蛋白质组学分析策略来鉴定奶牛发情期和发情间期的唾液蛋白质组学差异。从奶牛发情期和发情间期采集唾液，并进行 LC-MS/MS 蛋白质组学分析。在奶牛唾液中检测到 2842 种蛋白质，其中，在发情期和发情间期唾液中分别鉴定出 2437 种和 1428 种非冗余蛋白质。此外，发现 1414 种和 405 种唾液蛋白质分别特异于发情期和发情间期，而 1023 种蛋白质在两组中均有表达。在差异表达的蛋白质中，56 种蛋白质的表达下调（丰度比<0.5），40 种蛋白质的表达上调（丰度比>2），与发情间期相比，发情期唾液中的蛋白质表达差异显著。在发情期奶牛唾液中检测到的蛋白质，如 HSD17B12、INHBA、HSP70、ENO1、SRD5A1、MOS、AMH、ECE2、PDGFA、OPRK1、SYN1、CCNC、PLIN5、CETN1、AKR1C4、NMNAT1、CYP2E1 和 CYP19A1 等，在发情期奶牛的唾液样本中仅被检测到。在发情期奶牛唾液中检测到的大量蛋白质被发现参与代谢途径、PI3K-Akt 信号通路、Toll 样受体信号通路、类固醇生物合成途径、胰岛素信号通路、钙信号通路、雌激素信号通路、催产素信号通路、TGF-β 信号通路和卵母细胞减数分裂。另一方面，在发情间期奶牛的唾液中检测到的蛋白质主要参与代谢途径。总的来说，这些数据初步证明了奶牛发情周期不同阶段唾液蛋白质存在潜在差异。
