College of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.
State Key Laboratory of Organic Electronics and Information, Displays & Institute of Advanced Materials (IAM), Jiangsu Key, Laboratory for Biosensors, Nanjing University of Posts & Telecommunications, Nanjing 210023, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 5;322:124798. doi: 10.1016/j.saa.2024.124798. Epub 2024 Jul 10.
We propose a novel strategy for tailoring the structure of fluorescent molecules to achieve emission at the tail end of the NIR-II window. The favorable spectroscopic properties and low cytotoxicity of YNs make them powerful tools for bioimaging. Notably, YN-4 exhibits a brightness 2.5 times greater than YN-3, 6 times that of IR-783, and 5 times that of ICG. This enhanced brightness enabled high-resolution imaging of mouse thoracic and abdominal cavities, tumor vasculature, and real-time monitoring of gastrointestinal motility using YN-4. Furthermore, covalent grafting of glucose onto the YN-Glu scaffold significantly improved tumor-targeting capability and facilitated tracking of glucose metabolism. This work aims to extend the application of fluorescent molecule imaging beyond the NIR-IIa window.
我们提出了一种新的策略，通过调整荧光分子的结构来实现近红外二区尾部的发射。由于 YN 的光谱性质优良且细胞毒性低，使其成为生物成像的有力工具。值得注意的是，YN-4 的亮度比 YN-3 高 2.5 倍，比 IR-783 高 6 倍，比 ICG 高 5 倍。这种增强的亮度使得使用 YN-4 能够对小鼠的胸腔和腹腔、肿瘤血管和胃肠道蠕动进行高分辨率成像和实时监测。此外，将葡萄糖共价接枝到 YN-Glu 支架上显著提高了肿瘤靶向能力，并促进了葡萄糖代谢的追踪。本工作旨在将荧光分子成像的应用扩展到近红外二区以外。
