Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, 626005, Tamilnadu, India.
Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, 626005, Tamilnadu, India.
Protein Expr Purif. 2024 Nov;223:106556. doi: 10.1016/j.pep.2024.106556. Epub 2024 Jul 14.
The spike protein of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is responsible for infecting host cells. It has two segments, S1 and S2. The S1 segment has a receptor-binding domain (RBD) that attaches to the host receptor angiotensin-converting enzyme 2 (ACE2). The S2 segment helps in the fusion of the viral cell membrane by creating a six-helical bundle through the two-heptad repeat domain. To develop effective vaccines and therapeutics against COVID-19, it is critical to express and purify the SARS-CoV-2 Spike protein. Extensive studies have been conducted on expression of a complete recombinant spike protein or its fragments. This review provides an in-depth analysis of the different expression systems employed for spike protein expression, along with their advantages and disadvantages.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的刺突蛋白负责感染宿主细胞。它有两个部分，S1 和 S2。S1 部分具有受体结合域（RBD），可附着于宿主受体血管紧张素转换酶 2（ACE2）。S2 部分通过两个七肽重复域创建六螺旋束，帮助病毒细胞膜融合。为了开发针对 COVID-19 的有效疫苗和疗法，表达和纯化 SARS-CoV-2 刺突蛋白至关重要。已经对完整重组刺突蛋白或其片段的表达进行了广泛的研究。本综述深入分析了用于刺突蛋白表达的不同表达系统，以及它们的优缺点。
