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硫代磷酸酯修饰的酵母苯丙氨酸tRNA 3'末端的特异性重金属标记

Specific heavy metal labeling of the 3-'terminus of phosphorothioate modified yeast tRNAPhe.

作者信息

Szalda D J, Eckstein F, Sternbach H, Lippard S J

出版信息

J Inorg Biochem. 1979 Nov;11(3):279-82. doi: 10.1016/s0162-0134(00)80024-0.

DOI:10.1016/s0162-0134(00)80024-0
PMID:390097
Abstract

Yeast tRNAPhe containing a phosphorothioate modified -CS-CS-A terminus binds two moles of chloroterpyridineplatinum(II). This result was determined by titrating the tRNA with 3HPtCl] Cl, removing excess platinum by cation exchange chromatography, and determining the amount of bound platinum by radiocounting techniques. It has thus been established that adjacent phosphorothioate modified nucleotides can be labeled with an electron dense stain, a necessary requirement for electronmicroscopic sequencing of polynucleotides to become practical.

摘要

含有硫代磷酸酯修饰的-CS-CS-A末端的酵母苯丙氨酸转运RNA（tRNAPhe）能结合两摩尔的氯代三联吡啶铂（II）。这个结果是通过用3HPtCl]Cl滴定转运RNA、通过阳离子交换色谱法去除过量的铂以及通过放射性计数技术测定结合的铂的量来确定的。因此已经确定，相邻的硫代磷酸酯修饰的核苷酸可以用电子致密染色剂标记，这是多核苷酸电子显微镜测序变得切实可行的必要条件。

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