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用修饰的苯丙氨酸转运RNA（tRNAPhe）研究发现，tRNA对氨酰-tRNA合成酶的亲和力与氨酰化能力之间缺乏相关性。

Lack of correlation between affinity of the tRNA for the aminoacyl-tRNA synthetase and aminoacylation capacity as studied with modified tRNAPhe.

作者信息

Renaud M, Ehrlich R, Bonnet J, Remy P

出版信息

Eur J Biochem. 1979 Oct;100(1):157-64. doi: 10.1111/j.1432-1033.1979.tb02044.x.

DOI:10.1111/j.1432-1033.1979.tb02044.x
PMID:385310
Abstract

The interactions of several modified yeast tRNAPhe [tRNAPhe lacking 7-methylguanine; a fragment comprising about 3/4 of the whole molecule: tRNAPhe (18--76); tRNAPhe (18--76) lacking 7-methylguanine] with yeast phenylalanyl-tRNA synthetase were studied. Upon excision of the 5'-quarter of the tRNAPhe molecule, the residual fragment still tightly binds to the synthetase, but can no longer by aminoacylated. Surprisingly, upon removal of the 7-methylguanine base at position 46 in this fragment, althought the affinity drops by a factor 10, a significant aminoacylation is restored. These results are discussed in terms of molecular flexibility and a model is proposed for tRNA-enzyme interaction, involving multisite recognition.

摘要

研究了几种修饰的酵母苯丙氨酸转运核糖核酸[tRNA缺乏7-甲基鸟嘌呤；一个包含整个分子约3/4的片段：tRNA（18-76）；缺乏7-甲基鸟嘌呤的tRNA（18-76）]与酵母苯丙氨酰tRNA合成酶的相互作用。切除tRNA分子的5'-四分之一后，残留片段仍紧密结合合成酶，但不再能被氨酰化。令人惊讶的是，在该片段中去除第46位的7-甲基鸟嘌呤碱基后，虽然亲和力下降了10倍，但显著的氨酰化得以恢复。从分子柔韧性的角度讨论了这些结果，并提出了一个涉及多位点识别的tRNA-酶相互作用模型。

相似文献

1
Lack of correlation between affinity of the tRNA for the aminoacyl-tRNA synthetase and aminoacylation capacity as studied with modified tRNAPhe.用修饰的苯丙氨酸转运RNA（tRNAPhe）研究发现，tRNA对氨酰-tRNA合成酶的亲和力与氨酰化能力之间缺乏相关性。
Eur J Biochem. 1979 Oct;100(1):157-64. doi: 10.1111/j.1432-1033.1979.tb02044.x.
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Conformational activation of the yeast phenylalanyl-tRNA synthetase catalytic site induced by tRNAPhe interaction: triggering of adenosine or CpCpA trinucleoside diphosphate aminoacylation upon binding of tRNAPhe lacking these residues.由tRNAPhe相互作用诱导的酵母苯丙氨酰-tRNA合成酶催化位点的构象激活：缺乏这些残基的tRNAPhe结合时腺苷或CpCpA三核苷二磷酸氨基酰化的触发。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1606-8. doi: 10.1073/pnas.78.3.1606.
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Effect of excision of the Y-base on the interaction of tRNAPhe (yeast) with phenylalanyl-tRNA synthetase (yeast).Y碱基切除对酵母苯丙氨酸tRNA与酵母苯丙氨酰tRNA合成酶相互作用的影响。
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