Evaluation of different bermudagrass germplasm at physiological and molecular level under shade along longitudinal and latitudinal gradients.

Responses of turfgrass to shade vary in individual species, and the degree and quality of low light; therefore, the selection of low light tolerant cultivars of turfgrass is important and beneficial for turf management rather than other practices. The stolons of thirteen bermudagrass genotypes were planted with two treatments and three replications of each treatment to establish for one month in the Yangzhou University Jiangsu China greenhouse. The established plants were transferred outside of the greenhouse, and 50% shading was applied to them with a black net. After 30 days of stress treatment, the morpho-physiological and biochemical analyses were performed. The expression of genes such as HEMA, HY5, PIF4, and Cu/ZnSOD was assessed. Cynodon dactylon is a C and perennial that grows as lawn grass and is used as forage. Based on different indicator measurements, the most shade-tolerant germplasm was L01 and L06 along the longitudes and L09 and L10 along the latitudes. At the same time, L02 and L08 were more susceptible, respectively. However, germplasm showed greater tolerance in higher latitudes while longitudinal plants showed less stress response. The current study aimed (1) to screen out the most shade-tolerant Cynodon dactylon genotype among 13 along longitudinal and latitudinal gradients in China. (2) to examine morpho-physiological indicators of different bermudagrassgenotypes; (3) to evaluate if and how differences in various indicators of bermudagrass correlated with geographic region. This study will significantly advance the use of Cynodon germplasm in breeding, genomics, management, nomenclature, and phylogeographical study. It will decisively define whether natural selection and migration can drive evolutionary responses for populations to adapt to their new environments effectively.